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A kind of rhodococcus erythrococcus capable of degrading phthalates and its application

A technology of didecyl phthalate and phthalic acid, applied in Rhodococcus erythrococcus YC-YT1 and its application fields, can solve the problems of protein research, less enzyme gene research, harsh environmental conditions of industrial wastewater, etc., and achieve degradation The effect of broad spectrum and stable degradation ability

Inactive Publication Date: 2020-02-18
中国农业科学院研究生院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the research on the genetic mechanism of its degradation mainly focuses on the cloning and identification of the enzyme genes involved in the degradation from phthalic acid to protocatechol, and has not yet penetrated into the protein research; The enzyme genes involved in the two-step deesterification process of phthalic acid are less studied
Moreover, there is currently a lack of reports on the application in the actual environment, such as the harsh environmental conditions of industrial wastewater, high salt, extreme pH, rich types of pollutants, etc., all of which put forward higher requirements for the tolerance of microorganisms

Method used

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  • A kind of rhodococcus erythrococcus capable of degrading phthalates and its application
  • A kind of rhodococcus erythrococcus capable of degrading phthalates and its application
  • A kind of rhodococcus erythrococcus capable of degrading phthalates and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Isolation and Identification of Example 1 Rhodococcus Erythrococcus YC-YT1

[0033] 1. Isolation of strains

[0034] Activated sludge samples were collected in the canal near Wangtang Industrial Zone, Xinwei Village, Xili Town, Nanshan District, Shenzhen, Guangdong Province. Under aseptic conditions, inoculate 10g of activated sludge samples into 100mL of inorganic salt ion medium containing 100mg / L DEHP, and cultivate them at 30°C and 180rpm. After every 7 days of culture, take 5% of the culture volume and transfer it to fresh inorganic salt medium, and increase the concentration of DEHP by 100mg / L each time, and transfer continuously for 3 times until the concentration of DEHP in the inorganic salt medium increases to 300mg / L.

[0035] Streak the acclimatized bacteria solution onto an inorganic salt medium plate containing 100mg / L DEHP, and culture in a 30°C incubator for 3 days. Pick a single colony on the plate and culture it after streaking, repeating three tim...

Embodiment 2

[0045] The degradation performance test of embodiment 2 Rhodococcus rubrum YC-YT1

[0046] 1. Rhodococcus erythrococcus YC-YT1 is resistant to di(2-ethylhexyl) phthalate (DEHP), dipropyl phthalate (DPrP) and butylbenzyl phthalate at a concentration of 100mg / L. Dimethyl phthalate (BBP), Dimethyl phthalate (DMP), Diethyl phthalate (DEP), Dibutyl phthalate (DBP), Diamyl phthalate (DAP), Dihexyl phthalate (DHP), diheptyl phthalate (DHPP), dicyclohexyl phthalate (DCHP), dioctyl phthalate (DOP), di For the degradation ability of nonyl ester (DNP) and didecyl phthalate (DDP), see Figure 4 .

[0047] Determination of di(2-ethylhexyl) phthalate (DEHP), dipropyl phthalate (DPrP) and butyl phthalate in inorganic salt medium of Rhodococcus rubrum YC-YT1 by high performance gas chromatography Benzyl benzyl ester (BBP), dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP), dipentyl phthalate (DAP ), dihexyl phthalate (DHP), diheptyl phthalate (DHPP), dicyclohexyl ...

Embodiment 3

[0068] Example 3 Application of Rhodococcus Erythrococcus YC-YT1 in the Treatment of Wastewater Containing Various Phthalates

[0069] In the present embodiment, the inorganic salt ion medium (not sterilized) containing 70g / L NaCl is used as simulated wastewater (hereinafter referred to as "wastewater"), and a 5.0L fermenter (BioFlo 115, New Brunswick Scientific Co., NJ, USA ) for simulated wastewater treatment. Rhodococcus erythrococcus YC-YT1 was cultured in LB liquid medium to the logarithmic phase (OD 600 =0.8, the cell concentration is about 1.6×10 8 CFU / mL), adding the prepared bacterial solution to the wastewater to a final concentration of 8 × 10 7 CFU / L, 1.6×10 8 CFU / L, 3.2×10 8 CFU / L, 4.8×10 8 CFU / L, 6.4×10 8 CFU / L and 8.0×10 8 CFU / L wastewater (each treatment is 2L wastewater), and DEHP, DPrP, DBP, DOP and BBP were added to the wastewater at the same time to each concentration of 100mg / L, as a treatment group; at the same time, the same concentration of pollu...

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Abstract

The invention provides a Rhodococcus ruber capable of degrading phthalates and an application thereof. The bacterium is YC-YT1, and the preservation number is CGMCC No.13959, which can degrade 85% of 100 mg / L bis(2-ethylhexyl) phthalate contained in inorganic salts within 12 hours, and within 5 days Can degrade 100mg / L bis(2-ethylhexyl) phthalate, dipropyl phthalate, butyl benzyl phthalate contained in inorganic salt culture medium 100%; Phthalates are also highly degradative. The Rhodococcus rubrum YC-YT1 of the present invention has a wide tolerance range for salt ion concentration, pH and temperature, can be applied to bioremediation and industrial wastewater treatment of environmental pollution caused by phthalates, and has good economic value and application prospects.

Description

technical field [0001] The invention relates to the field of microorganisms and biodegradation, in particular to a Rhodococcus ruber (Rhodococcusruber) YC-YT1 and application thereof. Background technique [0002] Phthalate Acid Esters (PAEs for short), also known as phthalates, is a general term for esters formed by phthalic acid. Phthalates are a class of environmental endocrine disruptors with artificial synthesis as the main source. They are mainly used in the preparation of polyvinyl chloride materials and act as plasticizers. As a plasticizer, the content of PAEs in plastic products accounts for about 20% to 30%, or even as high as 50%. Since the advent of polyvinyl chloride (PVC) in the 1950s, PAEs have been produced on a large scale. At present, it occupies more than 80% share of the global plasticizer market. In addition to being plasticizers, PAEs are also widely used as raw materials for the production of paints, adhesives, insect repellants, cosmetics, fragran...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C09K17/14C12R1/01C02F101/34
CPCB09C1/10C02F3/34C02F2101/34C09K17/14C12N1/20C12N1/205C12R2001/01
Inventor 闫艳春杨婷任磊贾阳樊双虎
Owner 中国农业科学院研究生院
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