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Application of a kind of rhodococcus erythrococcus strain, bacterial preparation and its bacterial cell and extract

A technology of Rhodococcus rhodochrous and bacterial cells is applied in the field of microorganisms, which can solve the problems such as no application of Rhodococcus rhodochrous, and achieve the effect of improving the immune function of the body.

Active Publication Date: 2021-08-13
FUJIAN INST OF MICROBIOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still no report on the application of Rhodococcus in the field of immune function regulation

Method used

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  • Application of a kind of rhodococcus erythrococcus strain, bacterial preparation and its bacterial cell and extract
  • Application of a kind of rhodococcus erythrococcus strain, bacterial preparation and its bacterial cell and extract
  • Application of a kind of rhodococcus erythrococcus strain, bacterial preparation and its bacterial cell and extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, the isolation of bacterial strain FIM-12

[0020] (1) In the mangrove forest of Bamen Bay, Wenchang, Hainan, the surface sediment of the mangrove forest was collected. Specifically, about 10 cm of soil was collected with a sampling shovel, put into a sterile plastic sealed bag, and stored at low temperature; 5g of the soil sample was weighed and added to Put in 50mL sterile 50% old seawater Erlenmeyer flask, shake and let it stand for 30min, take the supernatant, that is, the original solution, and carry out gradient dilution, the selected dilution is 10 -2 、10 -3 、10 -4 and 10 -5 suspension;

[0021] (2) Take 0.1mL of the stock solution and the selected suspension and apply it on the ISP4 medium plate prepared by adding 50mg / L potassium dichromate water, repeat 3 parallel plates for each sample, and then spread the coated plate Culture at 32°C, observe the morphological characteristics of the colony appearance, size, color, edge shape, surface dry and...

Embodiment 2

[0023] Embodiment 2, identification of bacterial strain FIM-12

[0024] (1) Physiological and biochemical characteristics identification: The obtained strain FIM-12 was streaked on a nutrient agar medium plate and inserted into a cover glass, cultured at 32°C for 7 days, and observed with an optical microscope, transmission and scanning electron microscope. Morphological characteristics and its hyphae. The main morphology and physiological and biochemical characteristics of the bacterial strain FIM-12 were obtained as follows:

[0025] Gram-positive bacteria, not acid-resistant, do not form aerial hyphae, do not produce spores, colonies are round and opaque, surface wrinkles are rough and dry, orange or swallow red, pigment does not secrete into the medium, mycelium is initially filamentous, After that, the diaphragm is produced and then broken into a rod or spherical shape; sucrose, glycerin, fucose, raffinose, fructose, maltose, mannitol, dextrin, glucose can be used, and l...

Embodiment 3

[0029] Embodiment 3, the fermentation of rhodococcus thalline

[0030] Strain activation: the strain FIM-12 preserved in glycerol was transferred to the slant medium, and cultured in a constant temperature incubator for 7 days, and the culture temperature was 32°C;

[0031] Seed preparation: Inoculate the slanted bacterial lawn obtained from the activation of the strain FIM-12 into the seed medium (100mL seed medium in a 500mL Erlenmeyer flask), and cultivate at 28°C and 230r / min for 42h to obtain the seed solution;

[0032] Fermentation culture: Inoculate the prepared seed solution in the fermentation medium (1000mL fermentation medium in a 5000mL Erlenmeyer flask) with a 5% (v / v) inoculum, ferment and cultivate at 32°C and 230r / min for 120h, and ferment The liquid was centrifuged at 3000r / min for 30min, and the collected bacterial cells were purified, washed and centrifuged, and 35.2g of wet cells of Rhodococcus erythrococcus were harvested per 1000mL of fermentation broth, ...

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Abstract

The present invention provides a strain of Rhodococcus ruber, a bacterial preparation and the application of its cells and extracts. The strain is Rhodococcus ruber FIM-12, which was preserved in China Microorganism Culture Collection on April 03, 2019 General Microbiology Center of the Management Committee, the preservation number is CGMCC No.17525; it can be used for microbial fermentation to prepare Rhodococcus cells and their extracts: Rhodococcus ruber (Rhodococcus ruber) FIM‑12 is fermented and cultured to collect bacterial cells and wash with purified water Wet cells are obtained; the wet cells are repeatedly frozen and thawed combined with ultrasonic breaking, and then extracted with absolute ethanol to obtain the Rhodococcus erythrococcus extract. The rhodococcus erythrococcus strain of the invention can be applied to industrial fermentation production to prepare rhodococcus erythrococcus cells and extracts of the rhodococcus erythrococcus. The prepared product has the functions of improving immune activity and tumor inhibition, and can be used as a production strain for further research and development.

Description

【Technical field】 [0001] The invention relates to the field of microorganisms, in particular to a strain of Rhodococcus erythrococcus, a bacterial preparation and the application of the bacterial cells and extract thereof. 【Background technique】 [0002] Immune function refers to the body's defense against diseases under the interaction of lymphocytes, monocytes and other related cells and their products. Immunity is a function of maintaining the physiological stability and balance of the body, and it is produced in the long-term evolutionary process of human beings. With the pollution of the environment, the reduction of exercise, the acceleration of the pace of life and the change of living habits, the immune function of human beings is also declining. The rejuvenation of some senile diseases, such as diabetes and viral diseases, is closely related to the disorder or damage of internal regulatory functions and the decline of immune function. In recent years, low immune f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/741A61P37/04A61P35/00C12R1/01
CPCA61K35/74A61P35/00A61P37/04C12N1/205C12R2001/01
Inventor 张祝兰杨煌建连云阳严凌斌陈洲琴王德森程贤
Owner FUJIAN INST OF MICROBIOLOGY
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