Rhodococcus ruber strain, rhodococcus ruber bacterium preparation and application of thalli cells and extracts of rhodococcus ruber

A technology of Rhodococcus rhodochrous and bacterial cells, which is applied in the field of microorganisms and can solve problems such as no application of Rhodococcus rhodochrous

Active Publication Date: 2019-08-30
FUJIAN INST OF MICROBIOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is still no report on the application of Rhodococcus in the field of immune function regulation

Method used

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  • Rhodococcus ruber strain, rhodococcus ruber bacterium preparation and application of thalli cells and extracts of rhodococcus ruber
  • Rhodococcus ruber strain, rhodococcus ruber bacterium preparation and application of thalli cells and extracts of rhodococcus ruber
  • Rhodococcus ruber strain, rhodococcus ruber bacterium preparation and application of thalli cells and extracts of rhodococcus ruber

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, the isolation of bacterial strain FIM-12

[0025] (1) In the mangrove forest of Bamen Bay, Wenchang, Hainan, the surface sediment of the mangrove forest was collected. Specifically, about 10 cm of soil was collected with a sampling shovel, put into a sterile plastic sealed bag, and stored at low temperature; 5g of the soil sample was weighed and added to Put in 50mL sterile 50% old seawater Erlenmeyer flask, shake and let it stand for 30min, take the supernatant, that is, the original solution, and carry out gradient dilution, the selected dilution is 10 -2 、10 -3 、10 -4 and 10 -5 suspension;

[0026] (2) Take 0.1mL of the stock solution and the selected suspension and apply it on the ISP4 medium plate prepared by adding 50mg / L potassium dichromate water, repeat 3 parallel plates for each sample, and then spread the coated plate Culture at 32°C, observe the morphological characteristics of the colony appearance, size, color, edge shape, surface dry and...

Embodiment 2

[0028] Embodiment 2, identification of bacterial strain FIM-12

[0029] (1) Physiological and biochemical characteristics identification: The obtained strain FIM-12 was streaked on a nutrient agar medium plate and inserted into a cover glass, cultured at 32°C for 7 days, and observed with an optical microscope, transmission and scanning electron microscope. Morphological characteristics and its hyphae. The main morphology and physiological and biochemical characteristics of the bacterial strain FIM-12 were obtained as follows:

[0030] Gram-positive bacteria, not acid-resistant, do not form aerial hyphae, do not produce spores, colonies are round and opaque, surface wrinkles are rough and dry, orange or swallow red, pigment does not secrete into the medium, mycelium is initially filamentous, After that, the diaphragm is produced and then broken into a rod or spherical shape; sucrose, glycerin, fucose, raffinose, fructose, maltose, mannitol, dextrin, glucose can be used, and l...

Embodiment 3

[0034] Embodiment 3, the fermentation of rhodococcus thalline

[0035] Strain activation: the strain FIM-12 preserved in glycerol was transferred to the slant medium, and cultured in a constant temperature incubator for 7 days, and the culture temperature was 32°C;

[0036] Seed preparation: inoculate the slant lawn obtained by activating the strain FIM-12 into the seed medium (100mL seed medium in a 500mL Erlenmeyer flask), and cultivate at 28°C and 230r / min for 42h to obtain the seed liquid;

[0037] Fermentation culture: inoculate the prepared seed solution in the fermentation medium (1000mL fermentation medium in a 5000mL Erlenmeyer flask) with a 5% (v / v) inoculum, ferment and cultivate at 32°C and 230r / min for 120h, and ferment The liquid was centrifuged at 3000r / min for 30min, and the collected bacterial cells were purified, washed and centrifuged, and 35.2g of wet cells of Rhodococcus rhodococcus were harvested per 1000mL of fermentation broth, and the wet cell yield of...

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Abstract

The invention provides a rhodococcus ruber strain, a rhodococcus ruber bacterium preparation and an application of thalli cells and extracts of rhodococcus ruber. The strain is rhodococcus ruber (Rhodococcus ruber) FIM-12 which is preserved in the China General Microbiological Culture Collection Center on April 3, 2019, with the preservation number being CGMCC No.17525. The strain can be used forpreparing rhodococcus ruber cells and extracts of the rhodococcus ruber through microorganism fermentation: after the rhodococcus ruber (Rhodococcus ruber) FIM-12 is subjected to fermentation and culture, thalli cells are collected, the thalli cells are washed with purified water so that wet cells are obtained, the wet cells are subjected to repeated freeze-thawing combined with ultrasonic wall breaking, then extraction with anhydrous alcohol is performed, and the rhodococcus ruber extracts are obtained. The rhodococcus ruber strain can be applied to industrialized fermentation and production,the rhodococcus ruber thalli cells and the rhodococcus ruber extracts are prepared, the prepared products have the functions of increasing immunity activity and restraining effects, and the rhodococcus ruber strain can be used as a production strain for further research and development.

Description

[0001] 【Technical field】 [0002] The invention relates to the field of microorganisms, in particular to a strain of Rhodococcus erythrococcus, a bacterial preparation and the application of the bacterial cells and extract thereof. [0003] 【Background technique】 [0004] Immune function refers to the body's defense against diseases under the interaction of lymphocytes, monocytes and other related cells and their products. Immunity is a function of maintaining the physiological stability and balance of the body, and it is produced in the long-term evolutionary process of human beings. With the pollution of the environment, the reduction of exercise, the acceleration of the pace of life and the change of living habits, the immune function of human beings is also declining. The rejuvenation of some senile diseases, such as diabetes and viral diseases, is closely related to the disorder or damage of internal regulatory functions and the decline of immune function. In recent year...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74A61P37/04A61P35/00C12R1/01
CPCA61K35/74A61P35/00A61P37/04C12N1/205C12R2001/01
Inventor 张祝兰杨煌建连云阳严凌斌陈洲琴王德森程贤
Owner FUJIAN INST OF MICROBIOLOGY
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