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Polycyclic aromatic hydrocarbon degrading bacterium Q3 and application thereof

A polycyclic aromatic hydrocarbon and degrading bacteria technology, applied in the field of microbial engineering, can solve problems such as difficult to be degraded, complex structure of high-ring polycyclic aromatic hydrocarbons, and increased difficulty of microbial degradation

Inactive Publication Date: 2019-11-19
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In fact, PAHs in the polluted environment exist in a mixed form, and each PAH interacts with each other, which increases the difficulty of microbial degradation
Low-ring PAHs can generally be directly degraded as the only carbon source and energy source for strain growth, while high-ring PAHs are more difficult to be degraded due to their more complex structures and low bioavailability

Method used

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  • Polycyclic aromatic hydrocarbon degrading bacterium Q3 and application thereof
  • Polycyclic aromatic hydrocarbon degrading bacterium Q3 and application thereof
  • Polycyclic aromatic hydrocarbon degrading bacterium Q3 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Screening and isolation of bacterial strains

[0034] Taking the PAHs-contaminated soil of Beijing Coking Plant as the bacterial source, 10 g of soil was weighed, and 90 mL of sterile water was added to it at 180 r min -1 , shake in a shaker at 37°C for 3 h, and after standing still for 30 min, take 5 mL of the supernatant and inoculate it into 50 mg L of pyrene -1 In 45 mL of sterilized inorganic salt culture solution, 37°C, 180 rpm -1 Shaker culture. Transfer 5 mL of culture medium every 10 days to 45 mL of pyrene concentration gradient in the form of 100, 200, 300, 500 mg L -1递增 The fresh sterilized inorganic salt culture medium was enriched with PAHs degrading bacteria after 5 transfers. After taking 100 uL of the last enrichment culture solution and coating it on the inorganic salt solid plate, the plate was turned upside down in the beaker containing pyrene, and a layer of pyrene film was spread on the inorganic salt solid plate by sublimation method...

Embodiment 2

[0047] Embodiment 2: bacterial strain is to the degradation of pyrene

[0048] With the Q3 ( Rhodococcus rhodochrous ) were inoculated into LB medium to the logarithmic phase, discarded the supernatant after centrifugation, washed twice by adding 20 mL of sterile water, discarded the supernatant after centrifugation again, harvested the bacteria, and inoculated the bacteria suspension at 600 nm The absorbance value (OD 600 ) were adjusted to 1.0. Inoculate the bacterial suspension of the obtained pure strains (10% inoculum) into the inorganic salt liquid medium containing pyrene, so that the initial content of pyrene is 25, 50, 100, 200 mg L -1 , each sample was repeated 4 times, and a control group was set at the same time. After culturing in a shaker at 37°C and 180 r / min for 8 days, the residual pyrene concentration was measured respectively, and the degradation rate was calculated. The results are shown in Image 6 .

[0049] The results showed that the strain Q3 coul...

Embodiment 3

[0050] Embodiment 3: bacterial strain is to the degradation of PAHs

[0051] As described in Example 2, the Q3 bacterial suspension was prepared, and the resulting bacterial suspension was inoculated (10% inoculum) into inorganic salts containing phenanthrene, pyrene, benzo[b]fluoranthene and benzo[a]pyrene In the liquid medium, the initial concentrations of phenanthrene, pyrene, benzo[b]fluoranthene and benzo[a]pyrene in the medium were 50 mg L -1 , 50 mg L -1 , 10 mg L -1 , 50 mg L -1 , each sample was replicated 4 times, and a control group was set at the same time. After culturing in a shaker at 37°C and 180 r / min for 8 days, the concentrations of residual phenanthrene, pyrene, benzo[b]fluoranthene and benzo[a]pyrene were determined. Calculate the degradation rate. Degradation rate as Figure 7 shown.

[0052] According to the above experimental procedures, the strain Q3 was verified against phenanthrene, pyrene, benzo[a]pyrene, benzo[b]fluoranthene, benzanthracene, ...

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Abstract

The invention relates to the technical field of microorganisms, in particular to a polycyclic aromatic hydrocarbon degrading bacterium and application thereof in polycyclic aromatic hydrocarbon degradation. The degrading bacterium is Rhodococcus rhodochrous, belongs to gram-positive bacteria, and has a deposit number of CGMCC No. 16446. The degrading bacterium screened by the invention has a widerange of degradable substrates and strong degradation performance, can efficiently degrade various high- and low-cyclic polycyclic aromatic hydrocarbon monomers, and especially have strong tolerance to high-concentration pyrene solution. Meanwhile, the strain can grow normally under neutral and alkaline conditions and under a salt concentration of less than 8%. The strain can be used for removingor degrading various polycyclic aromatic hydrocarbon monomers, especially removing or degrading composite high-cyclic polycyclic aromatic hydrocarbons in soil and water environment.

Description

technical field [0001] The invention relates to the field of microbial engineering, in particular to a degrading bacterium Q3 for degrading polycyclic aromatic hydrocarbon pollutants and its application. Background technique [0002] Polycyclic aromatic hydrocarbons (polycyclic aromatic hydrocarbons, PAHs) refer to the compounds connected by two or more benzene rings in the form of condensed rings. Organic Pollutants. It has the characteristics of high toxicity, thermal stability, bioaccumulation, hydrophobicity, and not easy to degrade, and it is ubiquitous in the environment. A large number of researches have been carried out on the pollution of polycyclic aromatic hydrocarbons at home and abroad. [0003] The main remediation methods of PAHs polluted environment include physical remediation, chemical remediation and biological remediation. Both the physical remediation method and the chemical remediation method have the characteristics of fast treatment speed, high t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C02F101/32C12R1/01
CPCB09C1/10C02F3/34C02F2101/327C12N1/205C12R2001/01
Inventor 蔡超高秀荣张又弛廖用开
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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