Ovarian-cancer-targeted polypeptide

An ovarian cancer and targeting technology, applied in the field of protein peptides, can solve the problems of unassessed in vivo targeting properties of peptides for drug delivery, few reports on screening ovarian cancer targeting ligands, etc., and achieve good targeting Ability, small molecular weight of polypeptide, good tissue penetration effect

Inactive Publication Date: 2013-01-23
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Up to now, this technology has successfully screened targeting peptides for liver cancer, prostate cancer, breast cancer and other human tumors, but there are few reports on screening ovarian cancer targeting ligands
At present, only the groups of Renata Pasqualini and Wadih Arap have applied this technology to in vitro screening of patient ascites (Oncogene, 2004 (23): 8859-8867) and ovarian cancer cells (Cancer Res, 2006 (1): 34-40) to obtain targetable drugs. However, the above researches all focus on in vitro cell or protein research, and have not evaluated the in vivo targeting properties of the peptide and the ability to use it as a ligand for drug delivery.

Method used

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  • Ovarian-cancer-targeted polypeptide
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  • Ovarian-cancer-targeted polypeptide

Examples

Experimental program
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Effect test

Embodiment

[0023] (1) In vivo screening of phage display random dodecapeptide library and sequencing and analysis of phage monoclonal DNA

[0024] BALB / c nude mice aged 4-6 weeks were selected and inoculated with human ovarian epithelial cancer cells SKOV-3 in the armpit of their right forelimbs to establish a human ovarian cancer tumor model. Choose Ph.D-12 from New England Biolabs TM The peptide library, diluted in PBS, was injected into the tumor model and circulated for 15 minutes. After heart perfusion, the tumor tissue was recovered, homogenized, weighed, and the cells were lysed to recover the phages, which were amplified and put into the next round of in vivo screening. At the same time, the titer of the phages recovered in each round of screening was determined by the method described in the New England Biolabs company's experimental guide, and the monoclonal DNA of the phages randomly selected and amplified in the second and third rounds of screening results was extracted an...

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PUM

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Abstract

The invention discloses an ovarian-cancer-targeted polypeptide. The polypeptide is screened from a tumor model of ovarian cancer by using phage display peptide library technology and the amino acid sequence of the polypeptide is WSGPGVWGASUK. Identification proves that the polypeptide can distinguish ovarian cancer cells from normal cells and ovarian cancer cells from other cancer cells, and thus can be combined with ovarian cancer cells specifically and be effectively internalized by the ovarian cancer cells. Serving as a medicine carrier, the polypeptide can improve the absorption of medicines. After being injected into the tumor model through vines, the polypeptide can be enriched in the tumor area of the ovarian cancer. The polypeptide is a totally new polypeptide with invivo targeting capacity proved, and the polypeptide can be used as an ideal ligand to construct a targeting carrier for transmitting anticancer medicines.

Description

Technical field [0001] The present invention is a protein polypeptide technology field. It involves a polypeptide obtained by using phage display peptide library technology in the body of ovarian cancer tumor model, including the amino acid sequence of polypeptides and the corresponding nucleotide sequence. Background technique [0002] Ovarian cancer is a common gynecological tumor. The incidence rate is third in breast cancer and uterine carcinoma, and the viciousness is high. The mortality rate is second only to breast cancer, which seriously threatens women's life.Statistics show that in 2011, about 225,500 women around the world were diagnosed with ovarian cancer, and 140,200 women died of such diseases.For a long time, the growth of removal surgery, radiotherapy and chemotherapy has not improved the survival rate of patients with ovarian cancer, so it is urgently needed to develop more new technologies to solve this problem.Among them, the introduction of the concept of dru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08
Inventor 尹光福马楚颖魏延
Owner SICHUAN UNIV
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