Cordyceps militaris (L) link as well as preparation method and application thereof

A technology of Cordyceps militaris and silkworm chrysalis, which is applied in the field of bioengineering, can solve the problems of not paying attention to the breeding of silkworm chrysalis of host insects, affecting product quality and absorbability, and unfavorable industrial production, so as to improve antioxidant function, delicate hand feeling, and internal quality Improved effect

Active Publication Date: 2013-01-30
吉林省中晟制药有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But Chinese patent application 00130381.3 only pays attention to the breeding of Cordyceps militaris strain, does not pay attention to the breeding of host insect silkworm chrysalis, and the product parasitism rate is only 61.18%; The strain used has only carried out the activation culture of strain before birth, Resulting in a low yield; cultivating Cordyceps militaris with canned bottles has low work

Method used

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  • Cordyceps militaris (L) link as well as preparation method and application thereof
  • Cordyceps militaris (L) link as well as preparation method and application thereof
  • Cordyceps militaris (L) link as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0034] Example 1

[0035] The biological cocoons of tussah silkworms were stored in the middle and late November of that year. The storage temperature was -1~2°C, the storage humidity was 50~60%, and the storage thickness was 30cm. Pupal stage standard: pupae rate 90%, healthy pupae rate 94%.

[0036] 1. Treatment of silkworm chrysalis: 1-2 days before production, put the cocoon of tussah silkworm into the hole of the cocoon cutting machine about 0.1-0.5mm below the tip of the cutting knife, and then start to rotate the cocoon cutting device in the same direction, the cutting knife quickly Cut the cocoon layer, and the silkworm chrysalis will fall off the cocoon shell naturally into the chrysalis container. There are 200 chrysalis per tray, and no overlapping is allowed. Then, the silkworm chrysalis will be transferred to the disinfection room by a conveyor, and the surface of the chrysalis will be sprayed by an automatic sprayer first. Water for 2 minutes, then use a conve...

Example Embodiment

[0057] Example 2

[0058] Table 1: Comparative test of Cordyceps militaris obtained by different preparation methods

[0059]

[0060] Existing method 1: the method described in Chinese patent application 00130381.3;

[0061] Existing method 2: the method described in Chinese patent application 87106987.3.

Example Embodiment

[0062] Example 3 : Analysis of main nutritional components of Cordyceps militaris powder

[0063] 1) Test items: the contents of uridine, adenosine, adenine, cordycepin, and uracil in two samples of Cordyceps militaris powder.

[0064] Sample name: Cordyceps militaris powder A (prepared according to Example 1); Cordyceps militaris powder B (prepared according to the method recorded in Chinese patent application 00130381.3).

[0065] Sample source: Guangzhou Daguang Pharmaceutical Co., Ltd.

[0066] Detection instrument: Shimadzu CS-910 dual-wavelength thin-layer scanner;

[0067] Reagents: silica gel H, silica gel GF254 (Qingdao Ocean Chemical Plant); 95% ethanol, chloroform, isopropanol (Beijing Chemical Plant AR).

[0068] Reference substances: uridine, adenosine, adenine, and uracil (all imported from West Germany); cordycepin (imported from Japan) with a purity of over 98%.

[0069] Thin layer conditions: 33 grams of silica gel GF254 (add 1.8 grams of disodium hydroge...

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Abstract

The invention provides a cordyceps militaris (L) link as well as a preparation method and an application thereof. The preparation method comprises the following steps of: (1) injecting fugal liquid of cordyceps militaris (L) link into silkworm chrysalis of biological cocoons of tussah, and cultivating the fugal liquid for 7-10 days at a temperature of 16-18 DEG C to obtain stiff cocoons; (2) carrying out no-illumination cultivation on the stiff cocoons for 7-10 days to form fruit body primordium on the surface of cocoon bodies; and (3) carrying out illumination cultivation on the stiff cocoons for 10-15 days with illumination intensity of 800-1000 lux, and then carrying out natural light illumination cultivation on the stiff cocoons at daytime and illumination cultivation on the stiff cocoons at night with illumination intensity of 800-1000 lux until fruit bodies grow out ascocarps. According to the invention, the biological cocoons of tussah and strains of the rejuvenated cordyceps (L) link are selected for cultivating the cordyceps militaris (L) link with high yield and high quality, a superfine grinding technology is adopted to fundamentally improve the dispersibility, dissolubility and absorptivity of the cordyceps militaris (L) link, the original biological activity and nutritional ingredients of the cordyceps militaris (L) link are maintained, and the utilization rate of raw materials is greatly improved.

Description

technical field [0001] The invention belongs to the field of bioengineering, in particular, the invention relates to a kind of Cordyceps militaris and its preparation method and application. Background technique [0002] Cordyceps militaris belongs to the phylum Eumycota, the class Ascomycetes, the order Hypocreales, the family Clavcipitaceae, and the genus Cordyceps. The scientific name is Cordyceps miltaris (L) Link. Valuable medicinal fungi. Cordyceps militaris powder and Cordyceps militaris powder capsules are two first-class national traditional Chinese medicine new drugs, both of which obtained official production approval documents (2003B00975, 2003B00976) in April 2003, and the product production approval numbers are Guoyao Zhunzi Z20030034 and Guoyao Zhunzi Z20030035 . [0003] At present, there are problems in the selection of raw materials (cocoon species and strains) and the processing of Cordyceps militaris powder in the cultivation of Cordyceps militaris and ...

Claims

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Application Information

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IPC IPC(8): A01G1/04A23L1/28C05G3/00
Inventor 陈文展苑桂华
Owner 吉林省中晟制药有限公司
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