Method for building efficient regeneration system of Yunnan rhododendron

A technology for efficient regeneration and establishment of methods, applied in the direction of plant regeneration, horticultural methods, botanical equipment and methods, etc., to achieve the effects of developed root systems, robust plants and large volumes

Active Publication Date: 2013-02-13
YUNNAN JICHENG LANDSCAPE TECH CO LTD
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The research on the regeneration system of Rhodo

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  • Method for building efficient regeneration system of Yunnan rhododendron
  • Method for building efficient regeneration system of Yunnan rhododendron
  • Method for building efficient regeneration system of Yunnan rhododendron

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Embodiment Construction

[0036] The materials and reagents used in the following examples are all commercially available, and each example is a conventional method unless otherwise specified.

[0037] A method for establishing a high-efficiency regeneration system of Rhododendron yunnanensis provided by the invention, the specific implementation steps are as follows:

[0038] 1. Obtaining sterile vaccines

[0039] Take the semi-lignified stems of Rhododendron yunnanensis with full lateral buds, cut them into small sections with 1-2 lateral buds, rinse them with washing powder water after oscillating, sterilize under aseptic conditions, and use 0.12% mercury chloride in turn Soak in solution for 15 minutes, soak in sodium hypochlorite solution with a mass fraction of 2% for 8 minutes, shake constantly during this period, then rinse with sterile water for 3 to 5 times, cut off both ends of the stem and inoculate in MS + 6-BA (6-benzyl adenine) 1mg / L+ NAA (naphthalene acetic acid) 0.1 mg / L, induce late...

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Abstract

The invention provides a method for building an efficient regeneration system of Yunnan rhododendrons and belongs to the field of a plant biotechnology. The method uses upper-middle portion leaves of Yunnan rhododendron aseptic seedlings as a material, one or more of hormones TDZ, ZT, 2,4-D, NAA, IAA and casein hydrolysate with appropriate concentration are mainly provided based on a woody plant medium (WPM) culture medium, and complete plants are obtained through a direct leaf regenerating way and an indirect leaf regenerating way. The method builds the efficient regeneration system of the Yunnan rhododendrons for the first time, provides a good theoretical basis and an experimental basis for genetic engineering breeding work of the Yunnan rhododendrons and simultaneously promotes biotechnology breeding progress of the rhododendrons effectively.

Description

technical field [0001] The invention belongs to the field of rhododendron biotechnology breeding, and specifically relates to a method for establishing a high-efficiency regeneration system of rhododendron yunnanensis, which can be used for gene engineering of rhododendron yunnanensis and screening of asexual mutant lines. Background technique [0002] Yunnan Rhododendron (Rhododendron yunnanense ) is Rhododendronaceae, Rhododendron is a shrub or small tree, mainly distributed in Yunnan (west, northwest, north, northeast), southern Shaanxi, western Sichuan, northwest Guizhou, southeastern Tibet, and also distributed in northeastern Myanmar outside the country. Born in miscellaneous forests, shrubs, pine forests, pine-oak forests, spruce or fir forest margins on hillsides, 1600-4000 meters above sea level. [0003] In 2008, the present inventor introduced Rhododendron yunnanensis from Zhongdian, Dali, Shizong, and Liangwang Mountains in Yunnan to the resource garden (Kunming...

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Application Information

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IPC IPC(8): A01H4/00
Inventor 彭绿春解玮佳张艺萍瞿素萍李世峰苏艳宋杰
Owner YUNNAN JICHENG LANDSCAPE TECH CO LTD
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