Method for building efficient regeneration system of Yunnan rhododendron
A technology for efficient regeneration and establishment of methods, applied in the direction of plant regeneration, horticultural methods, botanical equipment and methods, etc., to achieve the effects of developed root systems, robust plants and large volumes
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[0036] The materials and reagents used in the following examples are all commercially available, and each example is a conventional method unless otherwise specified.
[0037] A method for establishing a high-efficiency regeneration system of Rhododendron yunnanensis provided by the invention, the specific implementation steps are as follows:
[0038] 1. Obtaining sterile vaccines
[0039] Take the semi-lignified stems of Rhododendron yunnanensis with full lateral buds, cut them into small sections with 1-2 lateral buds, rinse them with washing powder water after oscillating, sterilize under aseptic conditions, and use 0.12% mercury chloride in turn Soak in solution for 15 minutes, soak in sodium hypochlorite solution with a mass fraction of 2% for 8 minutes, shake constantly during this period, then rinse with sterile water for 3 to 5 times, cut off both ends of the stem and inoculate in MS + 6-BA (6-benzyl adenine) 1mg / L+ NAA (naphthalene acetic acid) 0.1 mg / L, induce late...
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