Fast propagation method of seedless roxburgh rose seedlings

Abstract

The invention relates to a vegetative propagation method of plant seedlings, and in particular relates to a fast propagation method of seedless roxburgh rose seedlings. The method comprises the following steps of: 1, an axillary bud induction culture initiating stage; 2, a rapid proliferation stage of axillary buds, wherein the extended axillary buds are cut into 2cm long stems with 1-3 axillae, the stems are inoculated to a proliferation culture medium for proliferation culture, the stems are cut into 2cm long stems with 1-3 axillae for proliferation induction of the axillary buds after growing to be more than 4cm high, and the process of the step 2 is repeated for realizing the vast proliferation of seedless roxburgh rose; and 3, a stage of tissue culture, rooting, seedling exercising and transplanting, wherein the obtained proliferated seedlings are inoculated to rooting media, the culture media on roots are cleared away after the root system grows to be 2cm-3cm, then the seedlings are transplanted to a seedling exercising substrate (with the ratio of humus to perlite being equal to 5:1), and finally the survival seedlings is transplanted to nutrition bags. The seedlings can be directly applied to forestation 4 months later after seedling exercising. The method is simple to operate, the proliferation coefficient is stable, and the quality of the seedlings is ensured, therefore, the method is capable of effectively meeting the requirements of industrialized production.

Description

technical field [0001] The invention belongs to a seedling production and application technology in plant tissue culture, and mainly relates to a method for rapidly cultivating tissue culture seedlings by repeatedly inducing axillary buds of Cili roxburghii. Background technique [0002] Seedless prickly pear ( Rosa sterilis ) commonly known as hook prickly pear, a unique species in Guizhou, is a climbing perennial evergreen shrub of the family Rosaceae, 4-6m high, and grows vigorously. Branchlets and petioles purplish red, with sparsely curved prickles, covered with off-white tomentose. Pinnate compound leaves with 7-9 leaflets, leaflets elliptic or obovate-elliptic, 1.5-3.5 cm long, 1-2.5 cm wide, apex acute or blunt, base nearly rounded, asymmetrical on both sides, Both sides glabrous, stipules epiphytic on petioles, lanceolate. 3-5 flowers form a corymb, terminal. Fruit oblate, about 2 cm in diameter, gray-brown, sparsely thorned. Ripe fruit is dark orange-yellow, a...

AI Technical Summary

Problems solved by technology

Seedless prickly pear is a wild resource with a narrow distribution range and few resources, so it cannot reproduce by seeds, so the natural reproduction is slow

Seedless Rosa roxburghii seedlings are propagated by traditional cutting methods, the propagation speed is relatively slow, and many materials are needed; especially for the propagation of excellent clone materials, the number of materials is small, and it is difficult to multiply in a large amount in a short period of time , cannot effectively meet the needs of enterprises and growers for high-quality seedlings. The reproduction coefficient of the tissue culture method is 3-4 times, which effectively solves the problem of rapid multiplication of excellent materials for seedless Rosa roxburghii, thus making excellent clones The material can be popularized in a large area, laying a solid foundation for high production