Method of producing glutathione through hansenula polymorpha fermentation

A technology of glutathione and Hansenula, which is applied in the field of industrial microorganisms, can solve problems such as high market prices, and achieve the effects of reducing production costs, increasing production, and improving processes

Inactive Publication Date: 2014-08-06
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, as an antioxidant, GSH is expensive in the market, and the main source of GSH in my country depends on imports. Therefore, it is urgent to independently develop a set of high-yield, low-cost, and low-energy GSH production methods.

Method used

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  • Method of producing glutathione through hansenula polymorpha fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 120

[0031] Example 120°C low-temperature batch fermentation without variable temperature control.

[0032] After activating the preserved strains at 30°C for 3-4 hours, take a ring of yeast cells and inoculate them into a 250ml Erlenmeyer flask containing 30ml of seed medium to cultivate the seed culture solution. The shaker speed is 110r / min, and the culture temperature is 37°C, culture time is 16h. The seed culture solution was inoculated into a 250ml Erlenmeyer flask containing 50ml fermentation medium according to the inoculum size of 10% (v / v) and fermented to obtain a fermentation solution. The shaking table speed was 110r / min, and the cultivation temperature was 20°C. The time is 36h. The DCW in the final product is 2.4g / L, the total amount of GSH reaches 26.74mg / L, and the intracellular GSH content reaches 21.11mg / g.

Embodiment 230

[0033] Example 230 ° C batch fermentation without temperature control.

[0034] During the fermentation process, the culture temperature was 30°C, the shaker speed was 110r / min, and the culture time was 36h. Others were the same as in Example 1. The DCW in the final product was 5.7g / L, and the total amount of GSH reached 66.96mg / L. The GSH content in it reaches 11.67mg / g.

Embodiment 3

[0035] Example 3 Fermentation in batches at 37°C without variable temperature control.

[0036] During the fermentation process, the culture temperature was 37°C, the shaker speed was 110r / min, and the culture time was 36h. Others were the same as in Example 1. The DCW in the final product was 2.7g / L, and the total amount of GSH reached 126.15mg / L. The GSH content in it reaches 46.151mg / g.

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Abstract

The invention provides a method of producing glutathione (GSH) through hansenula polymorpha fermentation. The optimum growth temperature of yeast cells and the optimum generation temperature of GSH are determined through a temperature gradient fermentation test according to the effect of the temperature on a cell metabolism process, and the production level of GSH is raised by reasonably regulating and controlling the optimum reaction temperature and adopting the temperature shift fermentation. The GSH fermentation technology is high in yield, low in cost and easy and simple to operate; the content of GSH in an end product can reach 216.68mg / L; the intracellular GSH content reaches 53.37mg / g; and compared with constant temperature cultivation, the content of GSH and the intracellular GSH content are increased by 41.78% and 13.53%.

Description

technical field [0001] The invention relates to the field of industrial microorganisms, in particular to a method for fermenting and producing glutathione by Hansenula yeast (H.polymorphaDL-1). Background technique [0002] Glutathione (GSH) is a biologically active tripeptide compound with γ-glutamyl and sulfhydryl groups formed by condensation of L-glutamic acid, L-cysteine ​​and glycine through peptide bonds. That is, γ-L-glutamyl-L-semi-glutamylglycine, the molecular formula is C 10 h 18 o 6 N 3 S, the relative molecular weight is 307.33, the melting point is 189°C-193°C (decomposition), the crystal is colorless, transparent, elongated columnar or plate shape, and the isoelectric point is 5.93. GSH exists in all biological cells and plays a direct or indirect role in many important biological phenomena, such as material transport, protein synthesis, metabolism and cell protection. With the deepening of research on GSH, the application of GSH in clinical and food fie...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12P21/02C12R1/78
Inventor钱卫东付云芳陈宣
OwnerSHAANXI UNIV OF SCI & TECH