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A detection kit for severe sepsis and acute liver failure
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A sepsis, kit technology, applied in the field of immunology
Inactive Publication Date: 2015-10-14
李海
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At present, there is no effective method that can effectively predict the outcome of the above diseases, but the commonly used method to find markers of disease outcome is to reflect the prognosis of the disease through the level of severity of immunosuppression (CARS severity)
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Embodiment 1
[0041] Example 1 Poor prognosis of acute severe liver failure patients with suppressed differentiation of monocyte subsets in vitro
[0042] Using the detection kit of the present invention, by detecting patient 1, male, 58 years old, his mononuclear cell differentiation ability (such as figure 1 As shown), the basic situation of the patient: the onset of the disease manifested as fatigue, poor appetite, yellow urine for 1 week, exacerbation with confusion for 1 day, no previous history of liver disease, prothrombin activity PTA was 27%, in line with acute severe liver disease Diagnostic criteria for failure. After the patient's first peripheral blood sample was collected (the first day after admission), 200 microliters of whole blood was collected and placed in a 96-well peripheral blood plate with a total of 3 wells, placed at 37°C, 5% CO 2 Incubate in the incubator of the whole blood sample, take the blood sample at 0 hours, double-stain with the flow cytometry antibody ...
Embodiment 2
[0043] Example 2 Patients with acute severe liver failure who successfully differentiated monocyte subsets in vitro have a good prognosis (survival):
[0044] Using the detection kit of the present invention, by detecting patient 2, male, 45 years old, the basic situation of the patient: the onset of the disease showed anorexia with dark urine color for 1 week, HBV was found to be positive 20 years ago, and the prothrombin activity PTA was 38%. Peripheral blood at 37°C, 5% CO 2 After incubation in the incubator for 36 hours, the component reagents in the diagnostic kit were detected and analyzed by flow cytometry, which showed that the mononuclear cells were successfully differentiated (Mo2 / Mo1 > 1). The analysis results showed that the prognosis was good, and the patient was finally treated by medical survived.
Embodiment 3
[0045] Example 3 Severe sepsis patients (liver is the first organ to fail) with poor prognosisin vitro monocyte subgroup differentiation suppression
[0046] Using the detection kit of the present invention, by detecting patient 3, male, 45 years old, his mononuclear cell differentiation ability (such as figure 2 As shown), the basic situation of the patient: the onset of anorexia accompanied by dark urine for 3 weeks, HBV positive was detected 20 years ago, and the prothrombin activity PTA was 34%. Due to liver failure, it was in line with severe sepsis blood standard. After the patient’s first peripheral blood sample was collected, 200 microliters of whole blood was taken and seeded into a 96-well peripheral blood plate, with a total of 3 wells, placed at 37°C, 5% CO 2 Incubate in the incubator of the whole blood sample, take the blood sample at 0 hours, double-stain with the flow cytometry antibody CD14 FITC and CD16 PE, and make the figure (1). During the incubation p...
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Abstract
The invention belongs to the technical field of the immunology, and relates to a detection kit for the severe septicopyemia and the acute liver failure. The detection kit comprises a sodium citrate contained anticoagulant tube, an erythrocyte lysate, a PBS solution, a CD45 human monoclonalantibody, a CD14 human monoclonalantibody, a CD16 human monoclonalantibody, and a 1% paraformaldehyde fixing solution. The detection kit can detect the peripheral bloods of septicopyemia and acute / acute-on-chronic liver failure patients through detecting the monocyte differentiation capability in order to determine the prognosis of the above diseases. Detection results show that the obstacles of the monocyte differentiation capability of the diseases still exist; and the peripheral blood monocyte differentiation repression is closely related with the disease prognosis because the mortality of monocyte differentiation repression patients obviously increases and the monocyte differentiation repression patients can survive after the disappearance of the differentiation repression. The detection kit provides positive meaningful reference data for further treatment schemes.
Description
technical field [0001] The invention belongs to the technical field of immunology, and relates to a detection kit for severe sepsis and acute liver failure. The detection of peripheral blood of patients with liver failure is used to judge the outcome of the disease. Background technique [0002] Severe sepsis (severe sepsis) and acute / acute on chronic liver failure (acute / acute on chronic liver failure) belong to the category of critical diseases, which are characterized by the failure of at least one major organ and severe The immune system is disturbed and the mortality rate is high. So far, there is no clinical diagnostic kit that can effectively measure the outcome of the disease. Medically, severe sepsis is defined as a patient with severe sepsis 48 hours after the first organ failure occurs on the basis of systemic systemic inflammatory response syndrome (SIRS) . Acute (Acute liver failure, ALF) / acute on chronic liver failure (ACLF) liver failure is defined as: pat...
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