Process of radix isatidis extract enzymolysis-alcohol precipitation for removing impurities

Abstract

The invention discloses a process of radix isatidis extract enzymolysis-alcohol precipitation for removing impurities. The process comprises the following steps: putting radix isatidis decoction pieces into an extracting tank, and adding water to decoct for two times, wherein the first time lasts for two hours, and the second time lasts for one hour; filtering a decocted solution and then concentrating the decocted solution with a double-effect vacuum concentrator; when concentrate to 1.15 of the relative density and the temperature is 50 DEG C, adding amylase with the dosage being 0.5 percent of the amount of medicinal materials, and continuing to concentrate; when the relative density is concentrated to 1.20 and the temperature is 50 DEG C, filtering, chilling, adding 95 percent of ethanol to cause the alcohol content to be 50 percent, standing for precipitation, and extracting supernate; recovering ethanol, and concentrating to 1.20 of the relative density when the temperature is 70 DEG C. Through enzyme-hydrolyzing and pasting starch, the process increases the yield of effective constituents and reduces the dosage of ethanol.

Description

technical field [0001] The invention relates to the technical field of removing impurities from Radix Radix Radix Extract, and more specifically relates to a method for removing impurities from Radix Radix Radix Extract by enzymolysis-alcohol precipitation. Background technique [0002] Isatis root extract is a plant extract extracted from the dried root of the cruciferous plant Isatis indigo, and its impurities are mainly starch. In the current technical scheme, the impurity removal method of Radix Radix Radix Extract adopts water extraction and alcohol precipitation method, takes Radix Radix Radix Radix, adds water to decoct, decocts and filters, and filtrate merges, concentrates to relative density and is 1.20 (50 ℃), adds ethanol to make The alcohol content is 60%, let it stand for precipitation, take the supernatant, recover the ethanol and concentrate it to an appropriate amount. From the perspective of explosion-proof safety, ethanol must be added to the medicinal li...

AI Technical Summary

Problems solved by technology

From the perspective of explosion-proof safety, ethanol must be added to the medicinal liquid only when the temperature drops to room temperature. However, due to the gelatinization of starch, the medicinal liquid becomes jelly-like. The precipitated gelatinized starch wraps the active ingredients, and there is a disadvantage that the amount of ethanol is large, and the active ingredients are easily wrapped and precipitated, resulting in a decrease in yield.

Others have studied the addition of amylase during the extraction process, but because the infiltration-dissolution-diffusion process of starch from the inside of the medicinal material tissue is far greater than the inactivation rate of amylase, the effect cannot be controlled

There are also people who study the extraction of Radix Radix Radix Extract by soaking at a temperature of 80 degrees. Although the leaching of starch is greatly reduced, the leaching of active ingredients is also greatly reduced, resulting in a waste of medicinal material resources.

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