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Microfluidic chip for researching interaction between cells and preparation method thereof

A microfluidic chip and cell technology, applied in biochemical equipment and methods, tissue cell/virus culture devices, biochemical instruments, etc., can solve problems such as no substantive breakthroughs, achieve good application prospects, and reduce consumption , the effect of simplifying the cell implantation process

Inactive Publication Date: 2013-04-17
SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, using a microfluidic chip to prepare microstructures and microchannels on its surface, relying on the laminar flow effect between multiple layers of liquid in the microchannel to drive the sample microfluidics, and to complete the implantation of various cells at the same time, currently in a variety of The application field of cell co-culture has not yet had a substantial breakthrough

Method used

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  • Microfluidic chip for researching interaction between cells and preparation method thereof

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Effect test

Embodiment 1

[0025] The microstructure and microchannel graphics of the two-layer chip of the centrifugal microfluidic chip were designed and drawn by computer-aided design software. The microstructure and microchannel of the two-layer disc-shaped polymethyl methacrylate (PMMA) chip were processed and prepared by the numerical control CNC system, and the chips of each layer were cleaned with tap water and distilled water, and the remaining fingerprints, oil stains and other stains on the chip surface were wiped with ethanol . On the double-sided adhesive film, the required microstructures and microchannels were processed with a cutting machine. The two-layer chip is carefully aligned, glued, and pressure-sealed to make a microfluidic chip for co-culture of two kinds of cells. Add cell culture solution containing normal cells and tumor cells from the left sample injection hole and right sample injection hole respectively, add cell culture solution from the middle sample injection hole, con...

Embodiment 2

[0027] The microstructure and microchannel graphics of the two-layer chip of the centrifugal microfluidic chip were designed and drawn with computer-aided design software. The microstructure and microchannel of the two-layer disc-shaped polymethyl methacrylate (PMMA) chip were processed and prepared by the numerical control CNC system, and the chips of each layer were cleaned with tap water and distilled water, and the remaining fingerprints, oil stains and other stains on the chip surface were wiped with ethanol . On the double-sided adhesive film, the required microstructures and microchannels were processed with a cutting machine. The two-layer chip is carefully aligned, glued, and pressure-sealed to make a multi-cell co-cultured microfluidic chip. Add cell culture solution containing normal cells and tumor cells from the left sample injection hole and right sample injection hole respectively, add cell culture solution from the middle sample injection hole, control the lef...

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Abstract

The invention relates to a microfluidic chip for researching the interaction between cells and the preparation method thereof. A microstructure and a micro channel are arranged on the surface of the microfluidic chip. The two types of cells are fixed in respective cell culture areas respectively. The cells interact with each other through the micro channel. The interaction between the two types of cells during the process of growth can be visually researched with a microscope, as a result, a new thought and a new research technique for researching cell biology are provided; and moreover, the microfluidic chip is mainly applicable to fields relevant to cell biology, genetics, drug screening and the like. The microfluidic chip achieves co-culture of two types of cells, has the characteristics of visuality, convenience, miniaturization, less dosage of agent and fewer samples, thereby providing a novel analyzing technology for co-culture of two types of cells.

Description

technical field [0001] The invention relates to a microfluidic chip for studying intercellular interactions and a preparation method thereof. The microfluidic chip has microstructures and microchannels on its surface, and two kinds of cells are respectively fixed in their specific cell culture areas. The channel carries out the interaction between cells, and the interaction between two kinds of cells in the growth process can be directly studied under the microscope, which provides new ideas and new research techniques for the study of cell biology, and is mainly used in cell biology, genetics and Drug screening and other related fields. Background technique [0002] In the late 1980s, in order to establish a culture system more similar to the in vivo environment and make the in vitro environment as consistent as possible with the in vivo environment, so that cells can communicate with each other and support each other for growth and proliferation, people based on cell cultu...

Claims

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Application Information

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IPC IPC(8): C12M3/00
Inventor 沙俊
Owner SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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