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A kind of collagen-based self-healing hydrogel and preparation method thereof

A self-healing and hydrogel technology, applied in the field of collagen materials, can solve the problems of non-self-healing, easy to be destroyed, endangering the health and safety of patients, etc., and achieve the effect of good toughness and self-healing, and a good cell living environment

Active Publication Date: 2021-05-25
FUJIAN UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the collagen hydrogel prepared by physical method, due to the weak intermolecular force, it is easy to be destroyed when subjected to external force; while the hydrogel formed by covalent bond cross-linking has a stable network structure and makes the gel The mechanical properties are improved, but at the same time, it also restricts the fluidity of the molecular chain, increases its brittleness, and it is difficult to repair itself after being damaged.
This non-self-healing property causes the collagen hydrogel implanted in the organism to break due to external force or other factors, and it is difficult to repair it artificially, thereby shortening the life of the hydrogel implant and endangering the health and safety of the patient.

Method used

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  • A kind of collagen-based self-healing hydrogel and preparation method thereof

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preparation example Construction

[0030] A preparation method of collagen-based self-repairing hydrogel, which comprises the following specific steps:

[0031] (1) Dissolve collagen in an acetic acid solution with a concentration of 0.01-0.1 mol / L, and then stir evenly to prepare a collagen solution with a mass concentration of 10-30 mg / mL;

[0032] (2) Pipette guar gum and dialdehyde guar gum at a ratio of 0.5 to 4:1 by mass ratio and dissolve them in deionized water at the same time, and then dissolve them in deionized water at 30 to 80 Stirring and mixing for 2 to 6 h under the temperature condition of ℃ to prepare a guar gum / dialdehyde guar gum compound solution with a total mass concentration of 10 to 30 mg / mL;

[0033] (3) Dissolving borax in PBS buffer solution to prepare a borax solution with a mass concentration of 0.05-0.2 mol / L;

[0034] (4) Blend the collagen solution prepared in step (1) with equal mass concentration and the guar gum / dialdehyde guar gum compound solution prepared in step (2) in p...

Embodiment 1

[0044] (1) Dissolve 5 g of guar gum in 50 mL of ethanol, add 40 mL of NaIO with a concentration of 0.1 mol / L 4 solution, and react in the dark at room temperature for 12 h; after the oxidation reaction is completed, add 0.5 mL ethylene glycol solution to the mixed solution to terminate the reaction, and stir at room temperature for 0.5 h; wash the reaction product 3 times with tert-butanol, wash After cleaning, it was subjected to vacuum freezing treatment for 12 h, and then the processed product was ground to obtain powdered formylated guar gum with a degree of formylation of 4%.

[0045] (2) Cut the cowhide into small pieces, put it into a meat grinder and grind it, then put the ground cowhide with a wet weight of about 10g into a 1 L Erlenmeyer flask, and add pepsin equivalent to 3% of the wet weight of the cowhide and 500 mL of acetic acid with a concentration of 0.1 mol / L, and then put it into a constant temperature shaker at 4 ℃ for vibration extraction at a speed of 180...

Embodiment 2

[0051] (1) Dissolve 10 g of guar gum in 50 mL of ethanol, add 40 mL of 0.5 mol / L NaIO 4 solution, and reacted in the dark for 24 h at room temperature; after the oxidation reaction was completed, 2 mL of ethylene glycol solution was added to the product to terminate the reaction, and stirred at room temperature for 2 h; Vacuum freeze treatment for 24 h, and then grind the processed product to obtain powdered formylated guar gum, with a degree of formylation of 8%;

[0052] (2) Cut the cowhide into small pieces, put it into a meat grinder and grind it, put the ground cowhide with a wet weight of about 10 g into a 1 L Erlenmeyer flask, add pepsin equivalent to 3% of the wet weight of the cowhide and 500 mL of acetic acid with a concentration of 0.5 mol / L, and then put it into a constant temperature shaker at 4 ℃ for vibration extraction at a speed of 180 r / min for 72 h. The solution extracted by shaking was centrifuged, salted out, and centrifuged again, and then dissolved in 0...

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Abstract

The invention discloses a collagen-based self-repairing hydrogel and a preparation method thereof. The collagen solution is uniformly mixed with a mixed solution of guar gum and dialdehyde guar gum, and then a borax solution is added for stirring and mixing, and vacuum dehydration is carried out. The solution of the present invention provides a feasible way and a new idea for the preparation of self-healing collagen-based hydrogel. In addition, the self-healing hydrogel prepared by the solution of the present invention can better meet the requirements of injectable The requirements of hydrogels, the structures destroyed during the injection process can be reconstructed in vivo; and self-healing hydrogels have unique advantages as three-dimensional cell culture carriers, which can better simulate the cell survival environment and help Contact information transmission between cells to realize co-culture of multiple and multi-layer cells.

Description

technical field [0001] The invention relates to the technical field of collagen materials, in particular to a collagen-based self-repairing hydrogel and a preparation method thereof. Background technique [0002] Collagen is the most abundant protein in animals, accounting for about one-third of the total protein in animals. Collagen's low antigenicity, good biocompatibility, and biodegradability make it widely used in various fields such as biomedical materials, drug delivery vehicles, tissue engineering, and cosmetics. In recent years, protein hydrogels, as an important class of biomaterials, have been widely used in the fields of biomedicine and tissue engineering. Protein hydrogel is a natural matrix with a three-dimensional network structure composed of protein molecules, water and cross-linking agents. Collagen hydrogels have similar properties to natural tissues and are biodegradable. Related research has attracted more and more researchers' attention, especially ge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08L89/00C08L5/00C08K3/38C08J3/075C08B37/00
CPCC08B37/0096C08J3/075C08J2389/00C08J2405/00C08K2003/387
Inventor 丁翠翠张敏兰飞龚凌诸郑志功
Owner FUJIAN UNIV OF TECH
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