Novel efficient micro-fluidic multi-cell co-culturing chip and preparation method thereof

A microfluidic chip and co-cultivation technology, which can be used in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc., can solve problems such as no substantial breakthroughs, achieve good application prospects, simplify Cell implantation process, the effect of reducing dosage

Inactive Publication Date: 2013-04-24
SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using a microfluidic chip to prepare microstructures and microchannels on its surface, relying on the laminar flow effect between multiple layers of liquid in the microchannel to drive the sample microfluidics, and to complete the implantation of various cells at the same time, currently in a variety of The application field of cell co-culture has not yet had a substantial breakthrough

Method used

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  • Novel efficient micro-fluidic multi-cell co-culturing chip and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The microstructure and microchannel graphics of the two-layer chip of the centrifugal microfluidic chip were designed and drawn by computer-aided design software. The microstructure and microchannel of the two-layer disc-shaped polymethyl methacrylate (PMMA) chip were processed and prepared by the numerical control CNC system, and the chips of each layer were cleaned with tap water and distilled water, and the remaining fingerprints, oil stains and other stains on the chip surface were wiped with ethanol . On the double-sided adhesive film, the required microstructures and microchannels were processed with a cutting machine. The two-layer chip is carefully aligned, bonded, and pressure-sealed to make a microfluidic chip suitable for co-cultivation of three types of cells, containing three inlets and one main channel for cell culture. Add three kinds of cell culture solutions containing different cells from the left cell mixed solution injection hole, the middle cell mi...

Embodiment 2

[0026] The microstructure and microchannel graphics of the two-layer chip of the centrifugal microfluidic chip were designed and drawn by computer-aided design software. The microstructure and microchannel of the two-layer disc-shaped polymethyl methacrylate (PMMA) chip were processed and prepared by the numerical control CNC system, and the chips of each layer were cleaned with tap water and distilled water, and the remaining fingerprints, oil stains and other stains on the chip surface were wiped with ethanol . On the double-sided adhesive film, the required microstructures and microchannels were processed with a cutting machine. The two-layer chip is carefully aligned, glued, and pressure-sealed to make a multi-cell co-cultured microfluidic chip. Add three kinds of cell culture solutions containing different cells from the left cell mixed solution injection hole, the middle cell mixed solution injection hole and the right cell mixed solution injection hole to control the l...

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Abstract

The invention relates to a novel efficient micro-fluidic multi-cell co-culturing chip and a preparation method thereof. A microstructure and a micro-channel are arranged on the surface of the micro-fluidic chip; the surface of the micro-channel is decorated by bovine serum albumin; under the driving of gravity generated by liquid level difference between a solution inlet and a solution outlet, three cells are implanted in different regions of the micro-channel by using laminar flow phenomenon between liquid flows; finally, the three cells are co-cultured to lay a basis for studies on cell biology; and the novel efficient micro-fluidic multi-cell co-culturing chip provided by the invention is mainly applied to the related field of cell biology, genetics, drug screening and the like. Through adoption of the micro-fluidic chip, the three cells are co-cultured; the micro-fluidic chip has the advantages of intuitive property, convenience, miniaturization and low consumption of reagents and samples; and a brand new cell culturing and analyzing technology is provided for multi-cell co-culturing.

Description

technical field [0001] The invention relates to a novel high-efficiency microfluidic multi-cell co-culture chip and a preparation method thereof. The surface of the microfluidic chip has a microstructure and a microchannel, and the surface of the microchannel is modified with bovine serum albumin. Driven by the gravity caused by the liquid difference between them, the laminar flow phenomenon between the liquid flows is used to realize the implantation of three kinds of cells in different areas of the microchannel, and finally realize the co-culture of the three kinds of cells, which lays the foundation for the study of cell biology. It is used in related fields such as cell biology, genetics and drug screening. Background technique [0002] In the late 1980s, in order to establish a culture system more similar to the in vivo environment and make the in vitro environment as consistent as possible with the in vivo environment, so that cells can communicate with each other and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
Inventor 叶嘉明王晓东沙俊聂富强
Owner SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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