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Microfluidic chip for multi-cell cocultivation and preparation method thereof

A microfluidic chip, cell culture technology, applied in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc., can solve problems such as no substantial breakthroughs, achieve good application prospects, reduce Dosage and simple operation

Inactive Publication Date: 2013-04-24
SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using a microfluidic chip to prepare microstructures and microchannels on its surface, relying on the laminar flow effect between multiple layers of liquid in the microchannel to drive the sample microfluidics, and to complete the implantation of various cells at the same time, currently in a variety of The application field of cell co-culture has not yet had a substantial breakthrough

Method used

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  • Microfluidic chip for multi-cell cocultivation and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0025] The microstructure and microchannel graphics of the two-layer chip of the centrifugal microfluidic chip were designed and drawn by computer-aided design software. The microstructure and microchannel of the two-layer disc-shaped polymethyl methacrylate (PMMA) chip were processed and prepared by the numerical control CNC system, and the chips of each layer were cleaned with tap water and distilled water, and the remaining fingerprints, oil stains and other stains on the chip surface were wiped with ethanol . On the double-sided adhesive film, the required microstructures and microchannels were processed with a cutting machine. The two-layer chip is carefully aligned, glued, and pressure-sealed to make a multi-cell co-cultured microfluidic chip. Add the cell culture solution containing cells from the left sample injection hole and the right sample injection hole respectively, and add the cell culture solution from the middle sample injection hole to control the liquid dif...

Embodiment 2

[0027] The microstructure and microchannel graphics of the two-layer chip of the centrifugal microfluidic chip were designed and drawn by computer-aided design software. The microstructure and microchannel of the two-layer disc-shaped polymethyl methacrylate (PMMA) chip were processed and prepared by the numerical control CNC system, and the chips of each layer were cleaned with tap water and distilled water, and the remaining fingerprints, oil stains and other stains on the chip surface were wiped with ethanol . On the double-sided adhesive film, the required microstructures and microchannels were processed with a cutting machine. The two-layer chip is carefully aligned, glued, and pressure-sealed to make a multi-cell co-cultured microfluidic chip. Add cell culture fluid containing different cells from the left, right and middle sample injection holes respectively, and control the liquid difference height between the left, middle and right sample injection holes and the wast...

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Abstract

The invention relates to a microfluidic chip for multi-cell cocultivation and a preparation method thereof. Microstructures and microchannels are arranged on the surface of the microfluidic chip; under the driving of gravity which is generated by liquid difference, the implantation of different areas of the microchannels of multiple cells is realized by using the laminar phenomenon; and finally, the cocultivation of the multiple cells is realized, so that the foundation is laid for the research of cytobiology; the microfluidic chip is mainly applied in the related fields, such as cytobiology, genetics, drug screening and the like. The microfluidic chip realizes the cocultivation of the multiple cells, has the characteristics of intuition, convenience, miniaturization and low dosage of reagent and samples, and provides a brand new analysis technology for the multi-cell cocultivation.

Description

technical field [0001] The invention relates to a microfluidic chip for multi-cell co-cultivation and a preparation method thereof. The microfluidic chip has microstructures and microchannels on its surface, and is driven by the gravity generated by the liquid difference to realize the laminar flow phenomenon of various cells. The implantation of different regions of the microchannel finally realizes the co-cultivation of various cells, laying the foundation for the study of cell biology, and is mainly used in related fields such as cell biology, genetics, and drug screening. Background technique [0002] In the late 1980s, in order to establish a culture system more similar to the in vivo environment and make the in vitro environment as consistent as possible with the in vivo environment, so that cells can communicate with each other and support each other for growth and proliferation, people based on cell culture technology Cell co-culture technology has been developed. C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
Inventor 沙俊
Owner SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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