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Coriolus versicolor strain and method for extracting Coriolus versicolor glucan by utilizing fermentation products of Coriolus versicolor strain

A technology of versicolor glucan and bacterial strains, applied in the field of microorganisms, which can solve the problems of high energy consumption and pollution, and achieve the effects of reducing consumption, being environmentally friendly, and having a high degree of instrument automation

Active Publication Date: 2013-05-01
西安恒田生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, the water extraction method consumes a lot of energy in the extraction process, while the acid extraction method and the alkaline extraction method cause serious pollution.

Method used

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  • Coriolus versicolor strain and method for extracting Coriolus versicolor glucan by utilizing fermentation products of Coriolus versicolor strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: A preparation method of Yunzhi glucan, the steps are as follows

[0023] (1) Inoculate the Yunzhi bacterial strain with the registration number of CGMCC No.6760 in the liquid medium for fermentation and culture for 4 days, then centrifuge the fermentation broth at 5000r / min for 10min to obtain Yunzhi mycelia; the liquid culture The base components are glucose 13.8g / L, sucrose 5g / L, yeast powder 2g / L, peptone 20g / L, KH 2 PO 4 2.5g / L, MgSO 4 ·7H 2 O 1.5g / L and VB 1 8mg / L; the fermentation culture conditions are 25°C, 120r / min;

[0024] (2) According to the weight ratio of 1:5, the mycelia of Yunzhi mycelium was added with a mass fraction of 2% Na 2 CO 3 In hot water at 60°C, extract for 1-2 hours, repeatedly extract and filter for 2-3 times, then combine the extracts and concentrate to 1 / 5-1 / 3 of the original volume;

[0025] (3) Take the extract by weighing and make water dilution in water of 20 times of volume, use 1.5% by weight of cellulase, 0.8%...

Embodiment 2

[0027] Example 2: A preparation method of versicolor glucan, the steps are as follows:

[0028] (1) Inoculate the Yunzhi strain with the deposit registration number of this application as CGMCC No.6760 in a liquid medium for fermentation and culture for 6 days, and then centrifuge the fermentation broth at 5000 r / min for 10 minutes to obtain Yunzhi mycelia. The fermented liquid contains glucose 13.8g / L, sucrose 5g / L, yeast powder 2g / L, peptone 20g / L, KH 2 PO 4 2.5g / L, MgSO 4 ·7H 2 O 1.5g / L and VB 1 8mg / L; the fermentation culture conditions are 30°C, 140r / min;

[0029] (2) The weight ratio is 1:5, and the mass fraction of Yunzhi mycelium is added with 2% Na 2 CO 3 In hot water at 60°C, extract for 1-2 hours, repeatedly extract and filter for 2-3 times, then combine the extracts and concentrate to 1 / 5-1 / 3 of the original volume;

[0030] (3) Take the extract by weighing and make the water dilution in 20 times the volume of water, use 2.0% cellulase, 1% papain, 3.0% b...

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Abstract

The invention relates to a preparation method for Coriolus versicolor glucan. The preparation method for the Coriolus versicolor glucan is characterized by comprising the following steps of: extracting Coriolus versicolor HT-6 (collection registration number: CGMCC (China General Microbiological Culture Collection Center) No.6760) mycelia through Na2CO3 and an enzyme-ultrasonic wave combined method; then, performing filtration and vacuum concentration on extracting solution; removing protein by using a Sevag method; performing decoloration treatment by using activated carbon; collecting supernate in the manner of centrifugal separation; performing sediment by using 95 percent of absolute ethyl alcohol; performing filter washing on precipitates by using absolute ethyl alcohol; and finally performing vacuum drying to obtain the Coriolus versicolor glucan. According to the preparation method disclosed by the invention, the extraction efficiency of the Coriolus versicolor glucan can be remarkably improved, and moreover, the operation time is obviously shortened, and the energy consumption is reduced. The preparation method is environmentally friendly; an adopted instrument is high in degree of automation and simple in operation, and therefore, the industrial production of the Coriolus versicolor glucan becomes possible.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular, the invention provides a new strain of versicolor versicolor and a method for extracting versicolor versicolor glucan from the fermentation product of the strain. Background technique [0002] Coriolus versicolor glucan has a variety of biological activities. Studies have shown that it has significant anti-tumor, anti-ulcer, liver and liver protection, anti-virus, blood fat-lowering and immune-regulating effects, and is widely used in clinical practice. The current Yunzhi glucan is all extracted from the fermentation product of the existing Yunzhi strain, and conventional extraction techniques such as water extraction, acid extraction and alkaline extraction are usually used at present. When using the existing Yunzhi strain and the existing extraction process, the highest yield of the extracted Yunzhi glucan usually does not exceed 10%. At the same time, the water extraction method c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C08B37/02
Inventor 唐满仓张鑫马玉明安德荣
Owner 西安恒田生物科技有限公司
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