Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of hyperin in preparing anti-ovarian-senescence drug

A technology of hyperin and ovarian aging, which is applied in the directions of drug combinations, pharmaceutical formulations, and medical preparations containing active ingredients to achieve the effects of expanding the scope of application, increasing the secretion of progesterone, and having obvious anti-apoptotic effects.

Inactive Publication Date: 2014-06-04
南京五岳生物医药科技开发有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the application of hyperin in the preparation of anti-ovarian aging drugs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of hyperin in preparing anti-ovarian-senescence drug
  • Application of hyperin in preparing anti-ovarian-senescence drug
  • Application of hyperin in preparing anti-ovarian-senescence drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] [Example 1] The proliferation effect of hyperoside on isolated ovarian granulosa cells (MTT method)

[0018] Female rats aged 22-27 days were killed by cervical dislocation after 48 hours of routine feeding. The bilateral ovaries of the rats were aseptically removed, and the ovaries were crushed to release granulosa cells. They were treated with 2 mL of DMEM-F12 containing penicillin and streptomycin. Dilute in culture medium and gently pipette to disperse into single cells. Pellet cells were collected by centrifugation. A certain concentration of cell suspension was inoculated in a 24-well plate, 80 μl per well, and 120 μl of culture medium was added to each well to adjust the cell density to 200 μl / well, and 6 parallel wells were set up for each group. Put it in 5% CO 2 Cultivate in the incubator for 24h. Take CO 2 The granule cell suspension was cultivated in the incubator for 24 hours, and each well was successively added with the concentration of cadmium chlori...

Embodiment 2

[0022] [Example 2] hyperoside on the estrogen and progesterone secretion effect of isolated ovarian granulosa cells (radioimmunization method)

[0023] Female rats aged 22-27 days were killed by cervical dislocation after 48 hours of routine feeding. The bilateral ovaries of the rats were aseptically removed, and the ovaries were broken to release granulosa cells. They were treated with 2ml of DMEM-F12 containing penicillin and streptomycin. Dilute in culture medium and gently pipette to disperse into single cells. Pellet cells were collected by centrifugation. A certain concentration of cell suspension was inoculated in a 24-well plate, 80 μl per well, and 120 μl of culture medium was added to each well to adjust the cell density to 200 μl / well, and 6 parallel wells were set up for each group. Put it in 5% CO 2 Cultivate in the incubator for 24h. Take CO 2The granule cell suspension was cultivated in the incubator for 24 hours, each well was successively added with cadmiu...

Embodiment 3

[0027] [Example 3] Effect of hyperoside on the secretion of estrogen and progesterone in natural aging rats (radioimmunoassay)

[0028] Adopt the pre-aging model of natural aging. Select 14-month-old female SD rats with a body weight of (270±30) g. After the animals are fed for one week, do vaginal cytology smears and observe 4 estrous cycles continuously. Vaginal cytology shows that the estrous cycle is prolonged and then continues to be in estrus , Repeated pseudo-pregnancy cell phase, as a successful model of female aging. The natural aging rats were randomly divided into model group, hyperin (10mg / kg, 30mg / kg, 50mg / kg) group and positive drug group, with 10 rats in each group, and another 11 female rats aged 4 months For the normal control group, it was intragastrically administered once a day. The normal control group and the model group were given equal volumes of normal saline. After 30 days of continuous administration, blood was collected from the orbital venous plex...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of hyperin in preparing an anti-ovarian-senescence drug. The hyperin can treat multiple ovarian senescence diseases, such as a polycystic ovarian syndrome, dysfunctional uterine bleeding, premature ovarian failure and a climacteric syndrome, and has a significant effect. According to the application, the hyperin is administrated orally, percutaneously or by injection for treating the ovarian senescence diseases.

Description

technical field [0001] The invention relates to the medical application of hyperin, that is, the application of hyperin in drugs for effectively treating various ovarian recession diseases. Background technique [0002] Ovarian aging is a complex biological process with multiple factors interacting and gradually accumulating. The main cause of ovarian aging is the decline in the number of follicles as the number of follicles passes by. The accumulation of metabolites in the body changes the microenvironment of the ovary, such as damage to follicles by free radicals and end products of glycation, and mitochondrial DNA mutations, With the continuous accumulation of factors such as telomere shortening, the quality of remaining follicles is also reduced; the expression and function of a series of genes in the ovary are changed, and the secretion of H-P-O axis (Hypothalamus-Pituitary-Ovary Axis, hypothalamus-pituitary-ovary axis) hormones Finely regulate ovarian function. The d...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/7048A61P15/00A61P15/12A61P5/34
Inventor 吕晔韦敏马红
Owner 南京五岳生物医药科技开发有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com