Growth-promoting antibacterial bacillus amyloliquefaciens sp., preparation method of conditioner and application

A technology of amylolytic spores and conditioners, which is applied in the application field of microorganisms in agriculture, and can solve the problems that restrict the sustainable development of facility production, frequent occurrence of soil-borne diseases, and imbalance of soil nutrients, and achieve high antibacterial and disease prevention effects. Wide antibacterial spectrum and low cost effect

Active Publication Date: 2013-05-01
INST OF AGRI ENVIRONMENT & RESOURCE SHANXI ACAD OF AGRI SCI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0002] With the rapid development of facility agriculture, continuous cropping and large-scale use of chemical fertilizers and pesticides lead to unbalanced soil nutrients, soil acidification, and frequent occurrence of soil-borne diseases, which seriously restrict the sustainable development of facility production.

Method used

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  • Growth-promoting antibacterial bacillus amyloliquefaciens sp., preparation method of conditioner and application
  • Growth-promoting antibacterial bacillus amyloliquefaciens sp., preparation method of conditioner and application

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preparation example Construction

[0038] (3) Preparation of solid conditioner Use various cheap and easy-to-obtain agricultural and sideline products as fermentation material: fermentation material components: bean curd powder: 30-33g, bran 25-30g, chaff 5-10g, bean cake powder 5-30g 10g, 12-20g corn flour, 20-30% water content; adjust the pH to be neutral; sterilize by autoclaving at 121°C for 1h. When it is cooled to about 45°C, add 3-10% seed liquid into the solid fermentation material. Static culture at 30~35℃ for 50~72h, the number of viable bacteria can be obtained ≥4*10 9 cfu / g. dry basal culture. It is a solid conditioner.

[0039] The characteristic of the production of the conditioner is that it uses the leftovers of agricultural and sideline products such as bean pods, bean stalks, and rice husks discarded during soybean harvesting. It is also cheap and environmentally friendly.

[0040] Three, Bacillus amyloliquefaciens PB-4 (T4,4) antibacterial spectrum in the present embodiment

[0041] The a...

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Abstract

The invention relates to bacillus amyloliquefaciens sp. Pb-4(T4,4), a preparation method of a conditioner, and an application. The bacillus amyloliquefaciens sp. is preserved in China General Microbiological Culture Collection Center (CGMCC), with the preservation number of CGMCC NO. 7155 and preservation date of January 18, 2013. The bacillus amyloliquefaciens sp. has obvious growth-promoting, antibacterial and disease-prevention effects on crops such as tomatoes, and can be colonized in soil and plants. The preparation method of the conditioner comprises the steps of liquid culture of bean product waste water and the like, and solid fermentation of soybean pod wall powder and the like, wherein a culture has viable bacteria as high as 4*10<9>cfu/ml and 5*10<9>cfu/g, and contains three growth-promoting antibacterial substances, namely antibacterial protein, enzyme and antibiotic. Waste recycling of the bean product waste water is realized, the cost is low, and the conditioner is suitable for industrial production, reduces or substitutes chemical pesticides, is safe and nontoxic, and meets the requirements of ecological agriculture and sustainable agricultural development.

Description

technical field [0001] The invention belongs to the application technology of microorganisms in agriculture, and mainly relates to a preparation method and application of a Bacillus amyloliquefaciens conditioning agent. Background technique [0002] With the rapid development of facility agriculture, continuous cropping and extensive use of chemical fertilizers and pesticides have led to imbalances in soil nutrients, acidification of the soil, and frequent occurrence of soil-borne diseases, which seriously restrict the sustainable development of facility production. [0003] At present, for soil restoration and plant disease prevention and control in facility production, there are many preparations using microbial production, such as: CN100999667 discloses a soil conditioner for facility vegetable fields and its preparation method and application, emphasizing that as a soil conditioner, bacteria The ratio of the bacteria in the agent and the ratio of the solid agent carrier,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01C1/00A01G13/00C12R1/07
Inventor 庞金梅焦晓燕武爱莲梁改梅
Owner INST OF AGRI ENVIRONMENT & RESOURCE SHANXI ACAD OF AGRI SCI
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