Method for improving dilinoleoyl phosphatidylcholine content with enzyme method

A technology of dilinoleoylphosphatidylcholine and content, which is applied in the field of phospholipids and can solve problems such as difficulty in DLPC content

Inactive Publication Date: 2013-05-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It is very difficult to further increase the content of DLPC by traditional methods
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Method used

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  • Method for improving dilinoleoyl phosphatidylcholine content with enzyme method
  • Method for improving dilinoleoyl phosphatidylcholine content with enzyme method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Dissolve 50g of PC-90 in 100ml of toluene, after completely dissolving, add 56g of linoleic acid and 10.6g of immobilized lipase Lipozyme TL IM with 2% water, mix well, and stir in a constant temperature water bath (300rpm ) 24h, the reaction finishes precipitating, adds chloroform-methanol (2 / 1, V / V) to extract target product, centrifugal (4000rpm) reclaims immobilized lipase, precipitating, precipitating after washing with acetone, obtains 35.2 grams of DLPC content as 60.4% (HPLC normalization) product.

Embodiment 2

[0020] Dissolve 50g of PC-90 in 184.2g of linoleic acid, add 10ml of chloroform, add 58.55g of immobilized lipase Lipozyme TL IM added with 5% water after complete dissolution, mix well, and stir in a constant temperature water bath at 60°C (300rpm) 48h, the reaction is completed and the precipitation is completed, and the target product is extracted by adding chloroform-methanol (2 / 1, V / V), and the immobilized lipase is recovered by centrifugation (4000rpm), precipitation, precipitation after washing with acetone, and 28.2 grams of DLPC are obtained. The product with a content of 60.9% (HPLC normalization method).

Embodiment 3

[0022] Dissolve 50g of PC-90 in 166ml of n-hexane, add 111g of linoleic acid after complete dissolution, add 32.2g of immobilized lipase Novozyme 435 added with 6% water, mix well, and stir in a constant temperature water bath under the condition of 55°C ( 300rpm) 36h, precipitation after the reaction, adding chloroform-methanol (2 / 1, V / V) to extract the target product, centrifugation (4000rpm) recovery of immobilized lipase, precipitation, precipitation after washing with acetone, to obtain 25.3 grams of DLPC The product with a content of 61.1% (HPLC normalization method).

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Abstract

The invention relates to a method for improving dilinoleoyl phosphatidylcholine (DLPC) content with an enzyme method. The invention belongs to the technical field of phospholipid. According to the invention, soybean PC-90 is adopted as a raw material; the raw material, linoleic acid and immobilized lipase are added to a certain organic phase, and an enzymatic acidolysis reaction is carried out; after desolvation, a target product is extracted by using chloroform/methanol (2/1, V/V); immobilized lipase is recovered by centrifugation; desolvation is carried out, and acetone washing and desolvation are carried out, such that the product with a DLPC content higher than 60% (HPLC normalization method) is obtained.

Description

technical field [0001] The invention discloses a method for enzymatically increasing the content of dilinoleoylphosphatidylcholine, which belongs to the technical field of phospholipids. technical background [0002] Phosphatidylcholine (DLPC) rich in linoleic acid has important biological functions. Studies have shown that polyene phospholipids (PPC) can effectively prevent and treat liver diseases and liver fibrosis, and can prevent cardiovascular diseases caused by arteriosclerosis . PPC not only affects the process of liver fibrosis, but also can restore the existing liver fibrosis. The principle is based on the fact that various liver diseases have damage to the ultrastructure of liver cell membranes and mitochondria, accompanied by loss of phospholipids, and insufficient cellular respiration and energy synthesis. One central step is to supplement exogenous phospholipids to enhance membrane fluidity and transmembrane transport system activity, so that damaged cell memb...

Claims

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Application Information

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IPC IPC(8): C12P13/00
Inventor 曹栋史苏佳曹沛
Owner JIANGNAN UNIV
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