Flax cerebroside compound and extraction and separation method thereof
A technology of lincerebroside and separation method, which is applied in the direction of steroidal compounds, chemical instruments and methods, sugar derivatives, etc., can solve the problems of inability to obtain production value, affect the in-depth research and deep processing of Weixian radish, and achieve the purpose of inhibiting cancer cells Effects of growth, blood pressure stabilization, and blood concentration improvement
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Embodiment 1
[0028] A method for separating and purifying linoleside compounds, the specific steps are as follows:
[0029] Soak and extract the dried and crushed Weixian radish or radish stem and leaf dry powder at room temperature for four times with methanol. The dry powder can be soaked for five days and then filtered to obtain a crude extract; the filter residue is soaked and filtered with the same amount of methanol as the previous time, and soaked four times, each time for five days; the four crude extracts are combined, vacuum reduced Concentrate under pressure to obtain a viscous dark green extract with a solid content of 30-60wt%.
[0030] The above-mentioned extract is diluted according to the ratio of 1g of extract to 2-5g of distilled water, extracted three times with petroleum ether, combined with the petroleum ether extract after petroleum ether extraction, concentrated under vacuum and reduced pressure to obtain the extract of petroleum ether, and poured Add petroleum ethe...
Embodiment 2
[0051] A method for separating and purifying linoleside compounds, the specific steps are as follows:
[0052] Soak and extract the dried and crushed Weixian radish or radish stem and leaf dry powder at room temperature for four times with methanol. The dry powder can be soaked for five days and then filtered to obtain a crude extract; the filter residue is soaked and filtered with the same amount of methanol as the previous time, and soaked four times, each time for five days; the four crude extracts are combined, vacuum reduced Concentrate under pressure to obtain a viscous dark green extract with a solid content of 50-60wt%.
[0053] The above-mentioned medicinal extract was diluted according to the ratio of adding 4 g of distilled water to 1 g of medicinal extract, extracted three times with petroleum ether, combined the petroleum ether extract after petroleum ether extraction, concentrated under vacuum and reduced pressure to obtain petroleum ether part extract, and added...
Embodiment 3
[0059] A method for separating and purifying linoleside compounds, the specific steps are as follows:
[0060] Soak and extract the dried and crushed Weixian radish or radish stem and leaf dry powder at room temperature for four times with methanol. The dry powder can be soaked for five days and then filtered to obtain a crude extract; the filter residue is soaked and filtered with the same amount of methanol as the previous time, and soaked four times, each time for five days; the four crude extracts are combined, vacuum reduced Concentrate under pressure to obtain a viscous dark green extract with a solid content of 30-50wt%.
[0061] After the above-mentioned extract is diluted according to the ratio of 1g of extract to 3g of distilled water, it is extracted three times with petroleum ether, and the petroleum ether extract after petroleum ether extraction is combined, concentrated under vacuum and reduced pressure to obtain extract of petroleum ether part, and added to the ...
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