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Method for detecting expression of bone sialoprotein (BSP) in brain glioma by utilizing high-throughput tissue chip

A tissue chip, glioma technology, applied in the fields of biochemical equipment and methods, biological testing, material testing products, etc., can solve the problem of no BSP prognostic value, etc., and achieve the effect of improving sensitivity

Inactive Publication Date: 2013-05-08
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no research on the expression of BSP and its prognostic value in glioma tissues

Method used

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  • Method for detecting expression of bone sialoprotein (BSP) in brain glioma by utilizing high-throughput tissue chip
  • Method for detecting expression of bone sialoprotein (BSP) in brain glioma by utilizing high-throughput tissue chip
  • Method for detecting expression of bone sialoprotein (BSP) in brain glioma by utilizing high-throughput tissue chip

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Embodiment 1

[0036] (1) Obtaining tissue samples

[0037] The protocol for this study and the acquisition of tissue samples were approved by the Biomedical Research Ethics Committee of the Second Military Medical University, Shanghai, China. Glioma tissue samples were derived from previously collected patient tissue samples from patients with glioma who underwent surgery in the Neurosurgery Department of Shanghai Changzheng Hospital between January 1999 and December 2008. Normal brain tissue samples were obtained from patients with severe brain trauma in which part of the normal brain tissue had to be removed to relieve intracranial pressure. These patients or their legal representatives had signed written informed consent for surgical treatment and allowed us to use the resected tissue samples. The selection criteria of patients in this study are as follows: 1. Diagnosed with primary glioma and no history of other tumors; 2. Complete clinical data, including age, sex, clinical manifestat...

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Abstract

The invention relates to a method for detecting expression of bone sialoprotein (BSP) in brain glioma by utilizing a high-throughput tissue chip. The method comprises the following steps of: (1) performing total RNA (ribonucleic acid) separation on brain tissues by using a Trizol reagent; (2) performing inverse transcription on the total obtained RNA to obtain cDNA (complementary deoxyribonucleic acid); (3) mixing the cDNA and SYBR fluorescent dye and carrying out amplification; (4) standardizing expression of IBSP through GAPD, and carrying out a comparison threshold cycle method by using an equation; and (5) establishing the tissue chip, performing immumohistochemical staining, performing statistic analysis on the immumohistochemical result, and detecting expression of BSP in the brain glioma. According to the method, the sensitivity of detecting the expression of BSP is greatly improved, and enough information can be acquired in few specimens; and moreover, the method has an important medical value for detecting the expression of BSP.

Description

technical field [0001] The invention belongs to the field of detecting the expression of BSP in glioma, in particular to a method for detecting the expression of BSP in glioma by using a high-throughput tissue chip. Background technique [0002] Glioma is the most common tumor in the central nervous system (CNS), 70% of which are malignant gliomas, and it is the most common and deadly tumor among tumors of CNS origin, with high recurrence and mortality rates. The most biologically aggressive subtype of glioma, glioblastoma multiforme (GBM, WHO grade IV astrocytoma), has a dismal prognosis. The current standard treatment regimen consists of maximal resection of the tumor followed by chemotherapy and radiation with the DNA alkylating agent, temozolomide. However, despite the above-mentioned effective treatment, the prognosis and survival period of GBM and anaplastic astrocytoma (WHO grade III) are still very short, and the median survival period is only about 14-16 months and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/68
Inventor 陈菊祥徐涛秦荣卢亦成严勇周劲旭王洪祥
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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