Tissue culture breeding method for hempleaf groundsel herb

A technology for tissue culture and soul-returning grass, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as shortening the cultivation cycle, and achieve the effects of shortening the growth cycle, high reproduction rate, and less material collection.

Inactive Publication Date: 2013-06-05
靖宇县蓝帝中草药种植有限公司 +1
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Problems solved by technology

[0003] In order to overcome the deficiencies in the existing artifical cultivation techniques of Revenant Grass, the present invention provides a breeding method of Revenant Grass tissue culture, which can not only improve the emergence rate of Revenant Grass, but also shorten the cultivation period

Method used

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Embodiment Construction

[0019] The present invention will be further described below in conjunction with specific examples.

[0020] The tissue culture breeding method of Resurrection Grass comprises the following steps:

[0021] (1) Medium preparation: select MS medium as the basic medium, 6-benzylaminopurine (6-BA), naphthaleneacetic acid (NAA), gibberellin, zeatin (ZT) and 3-indoleacetic acid (IAA ) as a regulatory hormone to prepare three culture media:

[0022] Primary differentiation medium A: MS+6-BA0.4mg / L+ gibberellin 3mg / L,

[0023] Secondary differentiation medium B: MS+6-BA0.5mg / L+ ZT0.5mg / L,

[0024] Rooting medium C: MS +NAA0.4mg / L+IAA0.5mg / L,

[0025] Add 5% sucrose and 1% agar to medium A and B, add 4% sucrose and 0.5% agar to medium C, adjust the pH value to 6.0 with 1mol / L NaOH and 1mol / L HCl, and put the medium A, B and C are sterilized in a high-pressure steam sterilizer at 0.1MPa and 120°C for 20min.

[0026] (2) Disinfection of explants: choose freshly germinated young leav...

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Abstract

The invention relates to a tissue culture breeding method for hempleaf groundsel herb. The tissue culture breeding method comprises the following steps of: with an MS culture medium as a basic culture medium, 6-BA, NAA, ZT, IAA and gibberellin as regulation hormone components and a newly germinated tender leaf of the hempleaf groundsel herb as an explant, disinfecting; inoculating the hempleaf groundsel herb explant to a primary differential medium to grow adventitious buds; transferring the explant with adventitious buds into a subculture differential medium; and opening an inoculation bottleneck and hardening seedlings, taking out tissue culture seedlings, transplanting the tissue culture seedlings into a mixed matrix culture cup, culturing for a period of time, and transplanting the tissue culture seedlings into a greenhouse, wherein the survival rate is 90-95%. The tissue culture breeding method provided by the invention has the advantages that few materials are adopted, cultivation is economical, culture conditions can be manually controlled, cultivation is not influenced by natural conditions, a growth cycle is short, reproduction rate is high and the quality of the hempleaf groundsel herb is guaranteed.

Description

technical field [0001] The invention relates to a method for breeding medicinal plants, in particular to a method for tissue culture and breeding of Herba Resurrection. Background technique [0002] Herb Resurrection is a perennial herb. The whole herb of the plant is used as medicine, the roots and rhizomes can be used as traditional Chinese medicine, and the flowers can be used as Mongolian medicine. It has the effect of relieving cough and eliminating phlegm. It is mainly distributed in the Changbai Mountain area of ​​Jilin Province. Due to the serious damage to the wild resources in recent years, coupled with continuous harvesting, the natural resources tend to be exhausted, so that the area and output of Resurrection Grass are declining year by year. However, the conventional methods of cultivating Resurrection Grass have the disadvantages of difficult seed treatment, low germination rate, and long cultivation period. Contents of the invention [0003] In order to o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 程钰翔王建华
Owner 靖宇县蓝帝中草药种植有限公司
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