Novel method, kit and application for inducing somatic reprogramming

A kit and application technology, applied in the field of inducing somatic cell reprogramming, can solve problems such as no reports of effects

Active Publication Date: 2013-06-05
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0016] Gata3, Gata6, Gata4, Pax1, Six1, Gata2, Foxa2 have been widely reported as mesendoderm differentiation genes, but their roles in the reprogramming process have not been reported so far

Method used

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  • Novel method, kit and application for inducing somatic reprogramming
  • Novel method, kit and application for inducing somatic reprogramming
  • Novel method, kit and application for inducing somatic reprogramming

Examples

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Embodiment Construction

[0047] The invention is further illustrated by the examples. Those of ordinary skill in the art can understand that the present invention is not limited to the embodiments, and those of ordinary skill in the art can modify the embodiments based on the teaching of the specification. These modifications are also included within the scope of the present invention as defined by the appended claims.

[0048] The experimental methods in the following examples are conventional methods unless otherwise specified.

[0049] 1. Mouse Strains

[0050] Transgenic mouse strain: TgN(GOFGFP)11Imeg(OG) was purchased from RIKEN BioResource Center. The mouse strain ICR was purchased from Victoria River.

[0051] 2. Cell Culture

[0052] Primary mouse embryonic fibroblasts (MEFs) and neonatal mouse skin cells (MDFs) were derived from ICR×OG transgenic mice. The culture medium for MEF and 293T cells was DMEM (Hyclone) medium containing 10% fetal bovine serum (FBS, Invitrogen). Mouse embryoni...

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Abstract

The invention provides a novel method, a kit and an application for introducing somatic reprogramming. The invention relates to an application of correlation factors for mesendoderm differentiation and development as inducing factors for producing induced pluripotent stem cells. The inducing factors are provided to differentiated cells so as to induce and produce the induced pluripotent stem cells (iPS cells), wherein the inducing factors includes the correlation factors for the mesendoderm differentiation and development, Sox2, Klf4 and optionally c-Myc. The iPS cells produced by using the correlation factors for the mesendoderm differentiation and development to replace Oct4 have similar characteristics with mouse embryonic stem cells, that is, the iPS cells have totipotency for maintaining self-renewal capability and differentiation.

Description

technical field [0001] The invention relates to a method for inducing somatic cell reprogramming, a kit and application. Background technique [0002] (1) Introduction to embryonic stem cells [0003] Embryonic stem cells (ESCs, referred to as ES cells.) Embryonic stem cells are a type of cells isolated from early embryos (before the gastrula stage) or primitive gonads. They have unlimited proliferation, self-renewal and multi-directional differentiation in vitro. characteristics. [0004] (2) Characteristics of stem cell pluripotency: [0005] Embryonic stem cells are called "pluripotent" stem cells because they have the ability to differentiate into three germ layer cells. In addition, embryonoma (EC) cells and embryonic germ (EG) cells have similar pluripotency to embryonic stem cells. The common characteristics of pluripotent stem cells are: ES cells have a morphological structure similar to that of early embryonic cells, with large nuclei, one or several nucleoli, mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10
Inventor 邓宏魁赵扬舒健吴晨吴业涛
Owner PEKING UNIV
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