Method for determining residual amount of dicamba in tobacco

Abstract

The invention discloses a method for determining the residual amount of dicamba in tobacco. The method comprises the steps of: extraction of a target object in tobacco, purification of N-propylethylenediamine, preparation of a standard stock solution and a standard working solution, and liquid chromatogram-tandem mass spectrum determination. The method disclosed in the invention overcomes the shortcomings of sample treatment methods in the prior art, optimizes pretreatment conditions directed to the tobacco sample, and optimizes the correlated detection conditions of LC-MS / MS (liquid chromatography-tandem mass spectrometry). Mainly, the ion source condition, the chromatographic column and the mobile phase system are optimized. Compared with traditional gas chromatography, the method employs a matrix dispersion solid phase extraction method to detect dicamba. The pretreatment process is simplified, and the analytical sensitivity is improved. Compared with the HPLC (high performance liquid chromatography) method, due to the employment of an ultra-high performance liquid chromatographic column RP18, the separation degree of the column is significantly improved, and the analysis time is significantly shortened. The use of the tandem mass spectrum makes the selectivity and sensitivity of the method improved, thus being more conducive to determination of low content herbicide residual.

Description

technical field [0001] The invention relates to the technical field of pesticide residue inspection, in particular to a method for measuring dicamba residues in tobacco. Background technique [0002] Dicamba was the first selective herbicide to be put into commercial production, and it is widely used in cereal crop fields, coniferous forests and pastures. Dicamba is a low-toxicity herbicide, which is absorbed and conducted through plant leaves, stems and roots, hindering the normal conduction of plant hormones and causing them to die. However, in the process of use, the results show that such substances can cause human soft tissue malignant tumors, and exhibit placental toxicity to animals. CORESTA's Agrochemical Advisory Committee (ACAC) set the limit of dicamba at 0.20mg / kg in the 118 kinds of agrochemical guidance residue limit table formulated in 2008. [0003] There have been relevant reports on the detection of dicamba residues in water, soil, vegetables and fruits, ...

AI Technical Summary

Problems solved by technology

[0004] Ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) is significantly superior to traditional methods in terms of sensitivity and selectivity in the detection of pesticide residues, and is superior to ordinary HPLC methods in terms of analysis speed, but there is currently no such detection method Application of detection of dicamba residues in tobacco

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