A liquid chromatography tandem mass spectrometry method for the determination of d-pantothenic acid content in urine
A technology of tandem mass spectrometry and liquid chromatography, applied in the field of D-pantothenic acid content, to achieve the effects of simple pretreatment, improved chromatographic separation process, and high sensitivity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
example 1
[0027] 1. Instruments and reagents: Agilent 1200 liquid chromatography (Agilent, USA), equipped with G1329A autosampler, G1311A quaternary mixing pump and G1316A, column oven; API 4000 triple quadrupole tandem mass spectrometer (Applied Biosystems, USA) ), equipped with electrospray ion source (ESI) and analyst 1.5 software data processing system.
[0028] The standard D-pantothenic acid was purchased from Wuhan Yitai Technology Co., Ltd. Shanghai Branch. The purity was greater than 98%; both formic acid and acetonitrile were chromatographically pure, and the water used in the experiment was deionized water.
[0029] 2. Sample treatment: Thaw urine at room temperature, then pipette 100 µL of urine into a 10 mL volumetric flask, dilute to volume with 0.1% formic acid aqueous solution, mix well, then pipette about 1 mL into a 1.8 mL chromatographic bottle , to be analyzed by LC-MS / MS.
[0030] 3. Determination method: draw standard solution (concentrations of standard workin...
example 2
[0031] Example 2: According to the determination method described in Example 1, 8 urine samples were selected, and the content of D-pantothenic acid was measured as shown in Table 2.
[0032] Table 2 The content level of D-pantothenic acid in samples (µg / mL)
[0033] serial number D-pantothenic acid serial number D-pantothenic acid 1 15.6 5 7.99 2 10.7 6 11.5 3 8.94 7 12.0 4 6.88 8 20.1
PUM
Property | Measurement | Unit |
---|---|---|
correlation coefficient | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com