Absolute quantification method of rat CYP450 enzyme mass spectrum
A technology of absolute quantification and mass spectrometry, which is applied in the field of proteomics, can solve problems affecting the accuracy of quantification, and achieve the effects of facilitating method promotion, sample processing and quantitative methods, and accurate measurement results
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[0053] Example 1: Determination of the content of five CYP450 enzyme subtypes in rat liver microsomal samples
[0054] Part 1: Preparation of Rat Liver Microsomal Samples
[0055] A. Administration induction process in rats
[0056] (1) Select two male rats with a body weight of 200g±10g, free to eat and drink;
[0057] (2) Purchase commercially available phenobarbital injection with a concentration of 100mg / ml and dilute to 15mg / ml with normal saline before use;
[0058] (3) The administration process is as follows
[0059] ① Phenobarbital induction group: intraperitoneal injection of 1 mL of phenobarbital in normal saline solution (15 mg / mL) for 3 consecutive days, once a day in the morning,
[0060] ②Blank control group: intraperitoneal injection of 1 mL of normal saline solution for 3 consecutive days, once a day in the morning;
[0061] B. Preparation of rat liver microsomes,
[0062] The two rats were operated as follows: the rats were killed by decapitation, the bl...
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