Method for preparing bioactive peptide for promoting growth of liver cells

A technology for promoting hepatocytes and active peptides is applied in the field of preparation of active peptides for promoting hepatocyte growth, which can solve the problems of poor stability and poor absorption effect, and achieve the effects of strong stability, function promotion and hepatocyte regeneration promotion.

Inactive Publication Date: 2013-06-19
黑龙江迪龙制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of poor absorption effect and poor stability of existing substance preparations for promoting liver cell regeneration, and to provide a preparation method of active peptides for promoting liver cell growth

Method used

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  • Method for preparing bioactive peptide for promoting growth of liver cells
  • Method for preparing bioactive peptide for promoting growth of liver cells
  • Method for preparing bioactive peptide for promoting growth of liver cells

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0019] Embodiment 1: The preparation method of the hepatocyte growth-promoting active peptide of this embodiment is carried out according to the following steps:

[0020] 1. Raw material pretreatment: take fresh healthy dairy cow liver, remove the blood vessels and fascia, rinse off the blood stains on the surface with purified water, and drain the water;

[0021] 2. Low-temperature water extraction: Homogenize the dairy cow liver treated in step 1 with a colloid mill at 0°C to 4°C, and add an equal volume of purified water at 4°C. Under the following conditions, use an ultrasonic crusher to sonicate at an ultrasonic frequency of 25,000 Hz for 15 minutes, and then place it at a temperature of 0°C to 4°C for 2 hours to obtain an extract;

[0022] 3. Heat treatment: Heat the extract obtained in step 2 to 80°C-82°C at a heating rate of 1-2°C / min through a water bath, keep it warm for 15 minutes, and then cool to 20°C-30°C;

[0023] 4. Centrifugation: centrifuge the heat-treated ...

specific Embodiment approach 2

[0029] Embodiment 2: This embodiment differs from Embodiment 1 in that: the temperature in step 3 is raised to 80° C., kept for 15 minutes, and then cooled to 25° C. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0030] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: in step 6, under the conditions of a temperature of 58° C. and a pressure of 150 Pa, the vacuum concentration is carried out to one third of the volume. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses a method for preparing bioactive peptide for promoting the growth of liver cells, and particularly relates to a method for preparing bioactive peptide for promoting the growth of the liver cells, and the method is used for solving the problems an existing material preparation for promoting liver cell regeneration is poor in absorption effect and stability. The method comprises the following steps of: 1, pretreating raw materials; 2, performing water extraction at low temperature; 3, performing heat treatment; 4, centrifugally collecting supernatant fluid; 5, performing ultrafiltration by adopting an ultrafilter, and collecting the supernatant fluid of which the molecular weight is less than 5000 daltons; 6, performing vacuum concentration; 7, performing ultrafiltration dialysis, and collecting the supernatant fluid of which the molecular weight is more than 1000 daltons; and 8, performing vacuum drying to prepare bioactive peptide powder for promoting the growth of the liver cells. The molecular weight of the small molecular polypeptide substance for promoting the growth of the liver cells is mainly distributed between 1000 and 3500, the absorption effect is good, and the stimulation index of the small molecular polypeptide substance is between 4 and 6. The bioactive peptide has very strong function of promoting the regeneration of the liver cells, is stable in 70% ethanol, cannot be precipitated by the ethanol, and has strong stability.

Description

technical field [0001] The invention relates to a preparation method of an active peptide for promoting liver cell growth. Background technique [0002] Liver cell growth-promoting active peptide is a small-molecule active peptide, which is a small-molecule peptide substance extracted from the liver of healthy dairy cows. It has the functions of promoting DNA synthesis and mitosis of liver cells, and promoting the repair of liver cells, especially It has obvious effects on liver cell regeneration and liver function recovery due to hepatitis, liver cirrhosis and liver surgery. [0003] As early as 1975, Labrecgue et al. reported that rat liver extract had the function of promoting liver cell regeneration. Later research confirmed that the substances that can promote liver cell regeneration are three types of proteins with different molecular sizes, namely large molecular weight (greater than 110,000 daltons), medium molecular weight (2-60,000 daltons) and small molecular wei...

Claims

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Application Information

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IPC IPC(8): C07K2/00C07K1/34
Inventor 董继胜王金富冯文善魏彦赵波
Owner 黑龙江迪龙制药有限公司
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