Application of suloctidil in preparation of cosmetics or medicine
A technology for cosmetics and drugs, applied in the field of medicine, can solve problems such as melanocyte apoptosis
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0033] Example 1 Qualitative observation of the inhibitory effect of sulodil on the formation of melanin in zebrafish
[0034] Zebrafish:
[0035] The zebrafish used in this example were provided by Hangzhou Huante Biological Breeding Fully Closed Circulation System Aquaculture System, and the breeding and use standards were strictly in accordance with the requirements of the American Laboratory Animal Care and Use Committee (IACUC).
[0036] The preparation method of fish breeding water: add 0.3g of sea salt (Instant Ocean salts) to 1L of reverse osmosis (RO) water.
[0037] Dimethyl sulfoxide (DMSO, analytically pure): purchased from Shanghai Jingchun Industrial Co., Ltd. Preparation of 0.1% DMSO solution (negative control): When in use, prepare a working solution with a concentration of 0.1% with pisciculture water, and prepare it for immediate use.
[0038] Arbutin (positive control): purchased from Shanghai Jingchun Industrial Co., Ltd. When used, 0.1% DMSO solution ...
Embodiment 2
[0045] Example 2 Quantitative evaluation of the inhibitory effect of sulodil on melanin formation in zebrafish
[0046] experimental method:
[0047] (1) Experimental grouping and embryo treatment: 210 well-developed zebrafish embryos were taken, and the embryonic development phase was 6 hpf (hour-postfertilization, hpf) after fertilization, and were randomly divided into 7 groups, as shown in the table below:
[0048]
[0049] The number of zebrafish embryos in each group was 30. During the operation, the embryos were evenly distributed to 48-well cell culture plates (Greiner, Germany), 15 embryos per well, 30 embryos were treated with each drug concentration, and 1 ml of water for embryo feeding was used in each well.
[0050] (2) Drug treatment: see operation step (2) of the experimental method in Example 1.
[0051] (3) Phenotype observation and quantitative statistics: The embryos treated with each drug concentration were photographed under a microscope at 48hpf to...
Embodiment 3
[0055] Example 3 Zebrafish melanin formation inhibition recovery experiment
[0056] experimental method:
[0057] (1) Experimental grouping and embryo treatment: 60 well-developed zebrafish embryos were taken, and the embryonic development phase was 6 hpf (hour-postfertilization, hpf) after fertilization, and they were randomly divided into two groups (negative control group and drug group). The number of embryos in each group was 30. During the operation, the embryos were evenly distributed to 48-well cell culture plates (Greiner, Germany), with 15 embryos in each well, and 1 ml of embryo breeding water in each well.
[0058] (2) Drug treatment: After the zebrafish embryos were treated for 42 hours according to the method of drug treatment in Example 1, the drugs were removed and placed in an incubator for 24 hours.
[0059] (3) Phenotype observation and statistics: observe the inhibition of melanin in each experimental group under a microscope and take pictures at 72hpf...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More