Lactobacillus buchneri for producing mannitol, and method for producing mannitol by fermentation

A technology of Lactobacillus Brucella and mannitol, which is applied in the field of microorganisms and achieves the effect of good implementation prospects

Active Publication Date: 2013-07-03
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Lactobacillus buchneri for producing mannitol, and method for producing mannitol by fermentation
  • Lactobacillus buchneri for producing mannitol, and method for producing mannitol by fermentation
  • Lactobacillus buchneri for producing mannitol, and method for producing mannitol by fermentation

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Embodiment Construction

[0022] Below in conjunction with the examples, the present invention is further described, the following examples are illustrative, not limiting, and the protection scope of the present invention cannot be limited by the following examples.

[0023] 1. Determination of raw materials

[0024] The raw material is taken from homemade sauerkraut in Northeast China. Bromocresol green is used as the chromogenic agent, combined with MRS medium formula to form a primary screening medium, and lactic acid bacteria are used to ferment glucose to produce acid. Bromocresol green turns yellow when it encounters acid, and lactic acid bacteria are screened out. Then use fructose as a substrate to ferment to produce products, and judge whether there are mannitol-producing lactic acid bacteria strains by high-performance liquid chromatography. The process is as follows:

[0025] (1) Preliminary screening of strains: After pretreatment, take 1 mL of sauerkraut juice and put it into a (25 mL / 250 ...

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Abstract

The invention relates to a lactobacillus buchneri for producing mannitol. The lactobacillus buchneri is called lactobacillus buchneri Y-4, named lactobacillus buchneri by classification, and preserved on 13, March, 2013 with preservation number of CGMCC NO.7300. The lactobacillus buchneri is characterized in that northeast sauerkraut is taken as the raw material, the lactobacillus buchneri is obtained by using an enrichment culture method, a preliminary screening method, a re-screening method and other methods for screening and purification, and the strain is determined as a new species according to the physiological and biochemical properties and 16 SrD DNA sequence analysis. The obtained strain has stable growth performance, the yield of mannitol is highest at a temperature of 30 DEG C and pH of 5.0, and the lactobacillus buchneri has a good research and application prospect.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to strain screening, in particular to a strain of mannitol-producing Lactobacillus brauchneri and a method for fermenting and producing mannitol. Background technique [0002] Mannitol, also known as mannitol and wood honey alcohol, is a hexavalent alcohol and is an isomer of sorbitol. Pure mannitol is colorless to white acicular or rhombohedral columnar crystal or crystalline powder, odorless, with cool and sweet taste, is the only non-hygroscopic crystal among polysaccharide alcohols, widely used in food, Medicine, light industry and chemical industry. [0003] At present, the widely used method for preparing mannitol is chemical synthesis, which is prepared by catalytic hydrogenation using sucrose, starch or glucose as raw materials. The hydrogenation of mannitol generally needs to be carried out under the conditions of high pressure and high temperature, metal catalysis and hydroge...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/18C12R1/225
Inventor 路福平李玉陈艳王洪彬王正祥
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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