The invention relates to a preparation method of a palladium-copper alloy film with controllable components and thickness, the palladium-copper alloy film and application. The preparation method comprises the steps that a layered plating method is adopted, metal palladium and copper are alternately deposited on a carrier to be placed layer by layer, the metal thickness and substance amount on each layer are controlled separately, finally, high-temperature alloying is carried out, and the compact palladium-copper alloy film is formed. According to the method, by controlling the quantity of electric charge of a system, metal palladium and copper with the corresponding volume are accurately deposited, and then the purpose of controlling the components of the alloy film is achieved. In addition, by adjusting and controlling the current density and controlling the film quality, quantities of electric charge in different stages are adjusted and controlled, the composition of the palladium-copper alloy film is controlled, and then the alloy film thickness is adjusted and controlled. Traditional chemical plating is broken through, the limit of metal reduction potential is broken through due to the limit that each layer needs the alloying step, and deposition of palladium metal on the copper-based surface is achieved. The preparation period of the alloy film is greatly shortened, the application range of the film is expanded, and the preparation method has the advantages of being simple in process and good in repeatability and is suitable for large-scale alloy film preparation.