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Method for preparing cell protein through fermentation of liquid waste in ginkgo leaf extract production

A cell protein and extract technology, applied in the field of waste resource utilization and agricultural product processing, to achieve good social and environmental benefits, low cost, and simple preparation methods

Inactive Publication Date: 2015-04-29
JIANGSU QIANYAOTANG GUOYI RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching, there is no report on the preparation of cell protein by fermentation of waste liquid in the production of ginkgo biloba extract (GBE) in the existing domestic and foreign patents or literature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Implementation of the first option

[0036] Collect 85 kg of waste liquid from the production of Ginkgo biloba extract after absorbing ginkgo flavonoids with macroporous adsorption resin, put it into a fermenter, heat and boil for 2 hours to sterilize, and when the temperature is lowered to 25-30°C, use dilute phosphoric acid (98% Dilute 1.5L of concentrated phosphoric acid with water to 40L), adjust the pH to 4.8, heat and boil for 1 hour to sterilize, and when the temperature drops to 36-38°C, add Aspergillus niger, Bacillus subtilis and brewer’s yeast in a weight ratio of 1:1 : 3 (ie 3 kg of Aspergillus niger, 3 kg of Bacillus subtilis, 9 kg of brewer's yeast) 15 kg of compound microbial bacterial starter, at a temperature of 36°C and a ventilation rate of 1:0.6v / v / min 1. Under the condition of stirring speed of 35r / min, after 22 hours of culture and fermentation, the culture and fermentation broth was dehydrated by disc centrifuge with a set speed of 6000r / min for 2...

Embodiment 2

[0038] Implementation of the second option

[0039] Collect 78.745 kg of waste liquid after absorbing ginkgo flavonoids by macroporous adsorption resin in the production of ginkgo leaf extract, put it into a fermenter, heat and boil for 2 hours to sterilize, and when the temperature is lowered to 25-30°C, add 5 kg of wort juice, 1 kg of ammonium sulfate, 0.015 kg of ferrous sulfate tetrahydrate, 0.04 kg of magnesium sulfate heptahydrate, 0.2 kg of calcium chloride dihydrate, stir evenly, and adjust the pH to 5.0 with dilute phosphoric acid (1.5L of 98% concentrated phosphoric acid is diluted with water to 40L) , then heated and boiled for 1 hour to sterilize, and when the temperature was lowered to 36-38°C, the composite microbial bacteria mixed with Aspergillus niger, Bacillus cereus and Candida tropicalis in a weight ratio of 1:1:3 were added 15 kg of starter, under the conditions of temperature 38°C, ventilation rate 1:0.6v / v / min, and stirring speed 40r / min, after 20 hours ...

Embodiment 3

[0041] Implementation of the third option

[0042] Collect 73 kg of waste liquid after the Ginkgo biloba flavonoids are absorbed by the macroporous adsorption resin in the production of Ginkgo biloba extract, put it into a fermenter, heat and boil for 2 hours to sterilize, and when the temperature drops to 25-30°C, add 12 kg of bran powder , stir evenly, adjust the pH to 4.8 with dilute phosphoric acid (1.5L of 98% concentrated phosphoric acid and dilute to 40L with water), heat and boil for 1 hour to sterilize, and when the temperature is lowered to 36-38°C, add Aspergillus niger and Bacillus licheniformis 15 kg of compound microbial bacterial starter mixed with Candida utilis in a weight ratio of 1:1:3, at a temperature of 37°C, a ventilation rate of 1:0.6v / v / min, and a stirring speed of 45r After 22 hours of culture and fermentation under the condition of 1 / min, the culture fermentation liquid was dehydrated by disc centrifuge with a set speed of 8000r / min for 18 minutes, a...

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PUM

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Abstract

The invention discloses a method for preparing cell protein through fermentation of liquid waste in ginkgo leaf extract production. The method comprises the following steps: putting the liquid waste adsorbed by a macroporous adsorption resin in the ginkgo leaf extract production into a fermentation tank, heating to be boiling for 2 hours, cooling to 25-30 DEG C, regulating pH to 4.8-5.2, then heating to be boiling for 1 hour for sterilizing, cooling to 36-38 DEG C, inoculating a composite microbial strain fermenting agent which is 15% of the weight of a culture solution, stirring for culturing for 18-22 hours at 36-38 DEG C and at a ventilation amount of 1: 0.6V / V / min, centrifugating for removing water and collecting a solid matter in a baking pan after the end of fermentation culture, and performing vacuum drying at 55 DEG C till the moisture content is less than 7% to obtain the cell protein product. In the method, the liquid waste adsorbed by the macroporous adsorption resin during decoction of ginkgo leaves for extracting ginkgo flavone is used; the method is suitable for industrial centralized processing; the utilization ratio of the liquid waste is high; in the preparation process, concentration is not required, so that energy consumption is low, the cost is low, economic and environmental benefits are high, and the implementation prospect is good; the cell protein is a scarce protein raw material at present, and has a very good market prospect.

Description

technical field [0001] The invention relates to the fields of waste resource utilization and agricultural product processing, in particular to a method for preparing cell protein by fermenting waste liquid produced in the production of ginkgo biloba extract. Background technique [0002] Ginkgo biloba extract is crushed from Ginkgo biloba leaves, heated and refluxed with ethanol, the extracts are combined, the ethanol is recovered and concentrated to an appropriate amount, added to the treated macroporous adsorption resin column, and eluted with water and different concentrations of ethanol in sequence , collect the corresponding eluate, recover ethanol, and spray dry; or recover ethanol, concentrate into a thick paste, vacuum dry, and pulverize to obtain (National Pharmacopoeia Committee, Pharmacopoeia of the People's Republic of China, 2010 edition, part one, page 392). However, the current manufacturers of Ginkgo biloba extracts have all optimized the extraction process o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/16C12N1/14C12R1/685C12R1/125C12R1/10C12R1/085C12R1/07C12R1/865C12R1/74C12R1/72
CPCC12N1/14C12N1/16C12N1/18C12N1/20
Inventor 张奎昌张志年
Owner JIANGSU QIANYAOTANG GUOYI RES INST CO LTD
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