Forest cultivation method of morchella crassipes
A technology for understory cultivation and morels, applied in the field of understory cultivation of morels thick-legs, can solve the problem of low yield of natural morels thick-legs, achieve significant economic and ecological benefits, increase production, and protect nature The effect of ecological environment
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Embodiment 1
[0030] Embodiment 1 The culture medium of thick leg Morchella fruiting body collection and mycelium separation, purification and use
[0031] Fresh and robust 8-9 mature fruiting bodies are taken from the seed field of Morchella. Be careful not to collect fruiting bodies in rainy days, so as not to increase the chance of contamination during transportation. Harvested fruit bodies were sorted, photographed, and dated prior to isolation.
[0032] The collected fruit bodies are placed in a ventilated box and immediately returned to the laboratory for isolation. The morel hyphae were isolated by tissue separation and spore separation, and purified and cultured.
[0033] The culture medium used for separation and purification is composed of the following components in parts by mass: 20 parts of potatoes, 2 parts of glucose, 3 parts of Morchella sprout humus, 2 parts of agar, and 100 parts of water.
Embodiment 2
[0034] The culture condition of embodiment 2 separation and purification of Morchella spp. mycelium
[0035] The mycelium was inoculated in a petri dish containing a culture medium. After the inoculation was completed, a label was attached, indicating the name, serial number and date of the strain, and placed in a constant temperature and humidity incubator for cultivation. The cultivation conditions were temperature 24 ℃, humidity 70 %, no light.
Embodiment 3
[0036] The cultivation of embodiment 3 rough leg Morchella solid culture strain
[0037] The mycelium of Morchella serrata is isolated and purified from the fruiting bodies of wild Morchella serrata. After the hyphae are separated and purified, thick, dense, vigorously growing, and uniformly distributed hyphae are selected to cultivate solid culture strains.
[0038] The bagged culturing material for culturing Morchella vulgaris solid culture strain is composed of the following components in parts by mass: 78 parts of sawdust, 15 parts of wheat bran, 3 parts of corn flour, 2 parts of sawdust flakes, 1 part of sucrose part, 1 part of quicklime, and the water content of the culture material is 55%-58%.
[0039] According to the formula of the culturing material, stir the miscellaneous sawdust, wheat bran, corn flour, and sawdust flakes evenly, then pour the melted sucrose, lime and water into the mixture, stir evenly, and the water content of the culturing material is 55%-58% %...
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