Tuna ground meat polypeptide angiogenesis inhibiting factor as well as preparation method and application thereof

An angiogenesis inhibition and tuna technology, which is applied to the preparation method of peptides, peptide/protein components, cardiovascular system diseases, etc., can solve the problems of high activity polypeptide angiogenesis inhibitors that have not been reported, and achieve easy monitoring and inhibition Angiogenesis, process science and reasonable effect

Inactive Publication Date: 2013-09-04
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the applicant found that the research on the preparation of polypeptide angiogenesis inhibitors by enzymatic hydrolysis technology using tuna leftovers and minced meat as raw materials is still in a blank stage, while the preparation of highly active polypeptides using enzymatic hydrolyzate of tuna leftovers and minced meat Angiogenesis-like inhibitory factors and their applications have not been reported

Method used

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  • Tuna ground meat polypeptide angiogenesis inhibiting factor as well as preparation method and application thereof
  • Tuna ground meat polypeptide angiogenesis inhibiting factor as well as preparation method and application thereof
  • Tuna ground meat polypeptide angiogenesis inhibiting factor as well as preparation method and application thereof

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Embodiment 1

[0030] 1) Take minced tuna and add K according to the ratio of material to liquid 1:3~1:5 2 HPO 4 -KH 2 PO 4 Buffer (pH 8.0), incubate at 38°C for 10 min, homogenate;

[0031] 2) According to the quality of tuna minced meat, add trypsin (trypsin), the amount of enzyme added is 2500 u / g, the enzymatic hydrolysis temperature is 38°C, and the enzymatic hydrolysis time is 5 hours; the enzymatic hydrolysis solution is heated to 90°C and kept at this temperature After 10 min, the enzyme was inactivated; the inactivated enzymatic hydrolyzate was centrifuged at 10,000 r / min for 20 min, and the supernatant was taken and freeze-dried to obtain the enzymatic hydrolyzate.

[0032] 3) The enzymatic hydrolysis product obtained in step 2) is subjected to ultrafiltration and chromatography in sequence to obtain the tuna minced meat polypeptide angiogenesis inhibitory factor, and its structure is determined by amino acid sequence analysis and mass spectrometry. The specific process is as fo...

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Abstract

The invention discloses a tuna ground meat polypeptide angiogenesis inhibiting factor as well as a preparation method and an application thereof. The amino acid sequence of the polypeptide angiogenesis inhibiting factor is Trp-Val-Thr-Gly-Ile (WVTGI), and ESI/MS (electrospray ionization/mass spectrometry) detection shows that a molecular ion peak (m/z) is 575.42Da ([M+H]+). The tuna ground meat polypeptide angiogenesis inhibiting factor Trp-Val-Thr-Gly-Ile (WVTGI) can be used for effectively inhibiting angiogenesis of chorioallantoic membrane (CAM) of chicken embryo and multiplication of prostate cancer cell DU-145. The tuna ground meat polypeptide angiogenesis inhibiting factor Trp-Val-Thr-Gly-Ile (WVTGI) can be used for preparing a medicine used for inhibiting angiogenesis and preventing and treating prostate cancer.

Description

technical field [0001] The invention relates to a tuna minced polypeptide angiogenesis inhibitor, a preparation method of the tuna minced polypeptide angiogenesis inhibitor, and a use of the tuna minced polypeptide angiogenesis inhibitor. Background technique [0002] Angiogenesis plays an important role in normal physiological processes such as human development and tissue repair, and is also one of the important pathological features of tumors. Folkman, a professor at Harvard Medical School in the United States, believes that the growth of tumors depends on the formation of new blood vessels, and the purpose of treating tumors can be achieved by inhibiting tumor angiogenesis. At present, this view has been confirmed by a large number of experiments, and tumor angiogenesis inhibitor (tumor angiogenesis inhibitor, TAI), a drug targeting blood vessels to treat tumors, has also become a research hotspot. [0003] Tuna is one of the important operating fish species of pelagic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K1/36C07K1/34C07K1/20C07K1/18C07K1/16C12P21/06A61K38/08A61P35/00A61P9/00
Inventor 张晨王斌罗红宇任西杰
Owner ZHEJIANG OCEAN UNIV
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