Method for preparing procalcitonin antibody

A procalcitonin, antibody technology, applied in the direction of calcitonin, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems of poor antibody affinity and specificity, and achieve optimal affinity and specificity, protein stability. , the effect of high protein yield

Active Publication Date: 2013-09-04
福建省洪诚生物药业有限公司
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Problems solved by technology

[0006] The embodiment of the present invention provides a preparation method of procalcitonin antibody, which is used to solve the technical problem of poor affinity and specificity of antibodies prepared from procalcitonin prepared by bacterial prokaryotic expression in the prior art

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  • Method for preparing procalcitonin antibody
  • Method for preparing procalcitonin antibody
  • Method for preparing procalcitonin antibody

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Embodiment Construction

[0023] In order to make the object, technical solution and advantages of the present invention clearer, the embodiments of the present invention will be further described in detail below in conjunction with the accompanying drawings. Here, the exemplary embodiments and descriptions of the present invention are used to explain the present invention, but not to limit the present invention.

[0024] The invention provides a preparation method of procalcitonin antibody. like figure 1 As shown, the method includes:

[0025] Step S110: constructing a procalcitonin recombinant plasmid;

[0026] In this step, firstly, the target fragment of the procalcitonin gene is prepared by using the primer sequence. According to the amino acid sequence provided by Genbank, the epitope analysis was carried out and combined with the gene sequence to determine that PCT76-252 was the target gene fragment, and the upstream and downstream primers introduced xho I and spe I restriction sites respecti...

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Abstract

The invention provides a method for preparing a procalcitonin antibody. The method comprises the steps of: building procalcitonin recombinant plasmids; with the procalcitonin recombinant plasmids as expression vectors, preparing the procalcitonin by eukaryotic expression of yeast; and preparing the procalcitonin antibody by utilizing the expressed procalcitonin protein. A monoclonal antibody is prepared from the procalcitonin in eukaryotic expression of yeast, so that the prepared monoclonal antibody has better affinity and specificity.

Description

technical field [0001] The invention relates to the field of biological material preparation, in particular to a preparation method of procalcitonin antibody. Background technique [0002] Human procalcitonin (PCT, Procalcitonin) comes from a single-copy gene located on chromosome 11. The gene consists of 2800 base pairs, including 6 exons and 5 introns, and the total length of the gene is about 7600 bases. After transcription, it is translated into procalcitonin precursor in the rough endoplasmic reticulum of parafollicular cells of the thyroid, including N-terminal 84 amino acids, active calcitonin (CT, 32 peptides) and calcitonin (katacalcin, 21 peptides) The three parts are connected by 2-peptide (-Lys-Arg-) and 4-peptide (-Gly-Lys-Lys-Arg-), respectively. Procalcitonin precursor generates PCT of 116 amino acids under the action of endogenous polypeptide enzymes. PCT is the hormonally inactive calcitonin propeptide substance. Under normal circumstances, calcitonin wi...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/26C07K14/585C12N15/81C12N15/16
Inventor 吴玉水黄庆生谢玉玲车速
Owner 福建省洪诚生物药业有限公司
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