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Method for purifying substrate mycelium from edible fungi mother culture

A purification method and edible fungus technology, which is applied in the field of edible fungus seed production, and can solve problems such as incomplete sterilization

Inactive Publication Date: 2013-09-11
邬金飞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the sterilization is not complete, there will be bacterial spores left in the culture medium, which will cause one-pot consequences

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] Mode 1: In this embodiment, the mycelia in the base of the Pleurotus ostreatus strain is purified, and the culture of the Pleurotus ostreatus test tube strain that is only contaminated with bacteria is continued. The total culture time is 20 days, so that the mycelia in the Pleurotus ostreatus culture medium can fully grow to the end department.

[0018] Purification method:

[0019] ① Disinfection: Put the inclined test tube to be inoculated, alcohol lamp, matches, inoculation shovel, large tweezers, alcohol cotton ball, waste plate, etc. on the ultra-clean workbench, turn on the ultraviolet lamp, and disinfect for 15-20 minutes;

[0020] ②Wipe and sterilize the mother seed test tube contaminated with bacteria repeatedly with alcohol cotton balls, put it into the ultra-clean workbench, and then sterilize the inoculation spatula and tweezers (especially the handle end) on the flame of an alcohol lamp;

[0021] ③ After the tweezers are cooled, use the handle end of the ...

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Abstract

The invention relates to a method for purifying substrate mycelium. In an edible fungi mother culture totally polluted by bacteria, the substrate mycelium is purified after fully growing, and a culture which is high in purity, pure in color, and exuberant in growth, and keeps culture original characters can be obtained again. The method for purifying the substrate mycelium is an effective rescue measure to prevent rare cultures from loss.

Description

technical field [0001] The content of the present invention relates to a method for purifying mycelia in the base of edible fungus mother species and belonging to the technical field of edible fungus seed production. Background technique [0002] Edible fungi use mycelium as strains. In the process of making strains, they often encounter the problem of parent species pollution. The main sources of pollution are mold and bacteria, which leads to the decline of strain performance. [0003] Because the color of mold hyphae is special, it is different from the color of edible fungus mycelium—white, so it is easy to identify after it contaminates the strains. After bacterial contamination of the strains, if you do not check in time at the beginning and remove the problematic test tube parent species, then when the edible fungus mycelium covers the bacterial colony, it will not be recognized on the outside, resulting in such a test tube. The mother species escapes the inspection ...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 邬金飞江伟
Owner 邬金飞
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