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Method for detecting and analyzing content of polyphenol in beer semi-finished product and bear finished product

An analysis method and technology for semi-finished products are applied in the detection of semi-finished beer, the analysis of polyphenol content of finished products, the analysis of polyphenol content in finished products, and the field of semi-finished products of beer. , difficult to find standard samples and other problems, to achieve good quantitative detection effect, simple production, high sensitivity

Active Publication Date: 2014-02-26
广州南沙珠江啤酒有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to purchase polyphenol standard samples. The polyphenol content of finished beer standard samples is low, and semi-finished products cannot be stored for a long time, and the polyphenol content cannot reach the upper limit of the detection range, so it is difficult to find suitable standard samples.

Method used

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  • Method for detecting and analyzing content of polyphenol in beer semi-finished product and bear finished product
  • Method for detecting and analyzing content of polyphenol in beer semi-finished product and bear finished product
  • Method for detecting and analyzing content of polyphenol in beer semi-finished product and bear finished product

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Sample

[0039] 1) Source of sample to be tested: beer finished product or fermentation liquid - fermentation liquid of large-scale beer production or fermentation liquid of laboratory fermentation bottle / tube;

[0040] 2) Sample pretreatment

[0041] Clarified samples are degassed by ultrasonic or vibration degassing method, and if there are turbid samples such as yeast, they are treated with filter paper or centrifugation.

[0042] 2. Detection and analysis method

[0043] 1) Preparation of tea polyphenol stock solution: Weigh about 2.5g of tea leaves, soak in 50mL of boiled distilled water for 20-30 minutes, then filter, and add the filtrate to 500mL of clarified beer.

[0044] 2) Test the polyphenol value of the tea polyphenol stock solution sample: test the tea polyphenol stock solution sample 6 times according to the GB / T4928 beer analysis method, the repeatability RSD is below 3%, and the average value is the polyphenol standard value of the tea polyphenol s...

Embodiment 2

[0057] 1. Sample

[0058] 1) Source of sample to be tested: beer finished product or fermentation liquid - fermentation liquid of large-scale beer production or fermentation liquid of laboratory fermentation bottle / tube;

[0059] 2) Sample pretreatment

[0060] Clarified samples are degassed by ultrasonic or vibration degassing method, and if there are turbid samples such as yeast, they are treated with filter paper or centrifugation.

[0061] 2. Detection and analysis method

[0062] 1) Preparation of tea polyphenol stock solution: Weigh about 4.5g of tea leaves, soak them in 50mL of boiling hot water and filter, add the filtrate to 900mL of finished beer, test it three times with the national standard method, and determine the standard value of polyphenols (usually about 300mg / L preparation), spare.

[0063] 2) Tea polyphenol standard diluent: Dilute the prepared tea polyphenol standard stock solution by 50mg / L, 100mg / L, 150mg / L, 200mg / L, 250mg / L, 300mg / L, and set aside. ...

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Abstract

The invention discloses a method for detecting and analyzing the content of polyphenol in a bear semi-finished product and a bear finished product. The method comprises the following steps of: (1) preparing a tea polyphenol stock solution preparation: soaking tea leaves by using heated distilled water, filtering, taking a clear solution, adding the clear solution into a beer, and mixing for later use; (2) detecting the polyphenol value of a tea polyphenol stock solution sample; (3) analyzing by using a chemical automatic analyzer: editing a program, uniformly mixing reagents, putting the sample into a reaction tank from a sample inlet, and reacting and analyzing by using a detector; (4) making a standard curve: diluting the tea polyphenol stock solution sample according to a certain concentration, and making a standard curve for a detection polyphenol module of the chemical automatic analyzer; (5) pre-treating a sample: degassing, filtering or centrifuging a sample to be detected; and (6) performing detection: detecting the sample to be detected in the detector. By adopting the detection and analysis method disclosed by the invention, an unstable polyphenol detection result caused by the fact that a standard substance is not provided for making a polyphenol standard curve for the chemical automatic analyzer can be avoided, and the method has the advantages of fastness, large sample amount, accuracy, high sensitivity and the like.

Description

technical field [0001] The invention relates to a method for analyzing polyphenol content in beer semi-finished products and finished products, belonging to the technical field of beer technology, in particular to an analysis method for detecting polyphenol content in beer semi-finished products and finished products by using a tea polyphenol addition method and using an automatic chemical analyzer . Background technique [0002] At present, the analysis methods of polyphenol content in beer are mainly manual methods, such as GB / T4928 beer analysis method and EBC analysis method of European Brewers Association. In recent years, some instruments have also been developed, such as chemical automatic analyzers and tannin meters, to realize the automatic analysis of polyphenol content, which is suitable for use in breweries with a large amount of detection. When the polyphenol content is detected by the instrument method, the standard curve is the key to the accuracy of the test...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N35/00
Inventor 周健莉钟丽琴潘立雄涂京霞梁宁
Owner 广州南沙珠江啤酒有限公司
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