Method for identifying mating type of protoplasted monokaryon of lepista sordida and special primer pair SR-6*4 thereof

A protoplast, monokaryotic technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., to achieve high accuracy and reliability
CN103305606BInactive Publication Date: 2015-03-11BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Publication Date
2015-03-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for identifying a mating type of protoplasted monokaryon of lepista sordida and a special primer pair SR-6*4 thereof. The method comprises the following steps of: by taking the genome DNAs of the protoplasted monokaryons of two plants of lepista sordida as templates, respectively, performing PCR (Polymerase Chain Reaction) amplification by using a PCR primer pair composed of two single-stranded DNAs as shown in SEQ ID No.1 and SEQ ID No.2; detecting the sizes of the PCR products obtained; if the PCR products of the protoplasted monokaryons of the two plants of lepista sordida to be identified both contain DNA segments of 500 bp-800 bp or not, indicating that the mating types of the protoplasted monokaryons of the two plants of lepista sordida to be identified are the same; if one plant contains the DNA segments of 500 bp-800 bp while the other plant contains no DNA segment of 500 bp-800 bp, indicating that the mating types of the protoplasted monokaryons of the two plants of lepista sordida to be identified are different.
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Description

technical field

[0001] The invention relates to a method for identifying the mating type of the protoplast monokaryon of the flower face mushroom and its special primer pair SR-6×4. Background technique

[0002] The nutrient incompatibility system is a genetic system for identifying aliens. In most fungi, this system can cause genetically distinct individuals to produce characteristic somatic incompatibility in the junction zone (Qi Yuancheng et al., Chinese cultivated pellagra Comparison of somatic cell incompatibility test and RAPD analysis results of Pleurotus species. Acta Mycophyta Sinica, 2010, 29 (3)) reaction, the intensity of somatic cell incompatibility reaction varies from individual to individual. In fungi, nutritional incompatibility is also known as somatic incompatibility or heterokaryotic incompatibility. fusion to maintain individual genetic stability. The mating type is the type of combination determined according to whether the individuals of the mating...

Claims

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