Method for improving germination rate and survival rate of sophora alopecuroide seeds and sophora alopecuroide seeds

A technology of sophora bean and survival rate, which is applied in the fields of seed and rhizome treatment, agriculture, and application, can solve the problems of seed coat pollution, labor and time consumption, low seed germination rate, etc. The effect of germination rate

Inactive Publication Date: 2013-10-02
SHANGQIU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the amount of wild resources of Sophora sophora in my country has dropped significantly. Although the seed setting rate of Sophora sophora per plant is high, the pods are not easy to crack and the seeds are not easy to scatter under natural conditions, and the hard seed rate is extremely high, which is not conducive to the normal germination and survival of seeds.
In addition, the seed coat is polluted by mold, which can easily lead to disease of young shoots
At presen...

Method used

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  • Method for improving germination rate and survival rate of sophora alopecuroide seeds and sophora alopecuroide seeds
  • Method for improving germination rate and survival rate of sophora alopecuroide seeds and sophora alopecuroide seeds

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Embodiment 1

[0023] The method for improving the germination rate and survival rate of wild medicinal plant Sophora sophora seed in the present embodiment, concrete steps are as follows:

[0024] (1) Selection of seeds: select 150 dried seeds with uniform size, uniform color and full grains;

[0025] (2) Sulfuric acid treatment: immerse the seeds in 80% concentrated sulfuric acid for 1.5 hours, filter out the sulfuric acid, and rinse the seeds with sterile water for more than 5 times;

[0026] (3) Ethanol treatment: immerse the sulfuric acid-treated seeds in 95% ethanol for 30 seconds;

[0027] (4) Sodium hypochlorite treatment: immerse the ethanol-treated seeds in a sodium hypochlorite solution with an available chlorine content of 3% for 8 minutes, and then rinse with sterile water for more than 5 times for later use;

[0028] Seed imbibition and germination adopt the following steps: place the above-mentioned treated seeds in a sterile container covered with filter paper, disperse the ...

Embodiment 2

[0032] The method for improving the germination rate and survival rate of wild medicinal plant Sophora sophora seed in the present embodiment, concrete steps are as follows:

[0033] (1) Selection of seeds: select 150 dried seeds with uniform size, uniform color and full grains;

[0034] (2) Sulfuric acid treatment: immerse the seeds in 90% concentrated sulfuric acid for 1.2 hours, filter out the sulfuric acid, and rinse the seeds with sterile water for more than 5 times;

[0035] (3) Ethanol treatment: Submerge the sulfuric acid-treated seeds in 95% ethanol for 22 seconds;

[0036] (4) Sodium hypochlorite treatment: immerse the ethanol-treated seeds in a sodium hypochlorite solution with an available chlorine content of 5% for 5 minutes, and then rinse them with sterile water for more than 5 times for later use;

[0037] Seed imbibition and germination adopt the following steps: place the above-mentioned treated seeds in a sterile container covered with filter paper, dispers...

Embodiment 3

[0040] The method for improving the germination rate and survival rate of wild medicinal plant Sophora sophora seed in the present embodiment, concrete steps are as follows:

[0041] (1) Selection of seeds: select 150 dried seeds with uniform size, uniform color and full grains;

[0042] (2) Sulfuric acid treatment: immerse the seeds in 98% concentrated sulfuric acid for 1 hour, filter out the sulfuric acid, and rinse the seeds with sterile water for more than 5 times;

[0043] (3) Ethanol treatment: immerse the sulfuric acid-treated seeds in absolute ethanol for 18 seconds;

[0044] (4) Sodium hypochlorite treatment: Submerge the ethanol-treated seeds in a sodium hypochlorite solution with an available chlorine content of 6% for 3 minutes, and then rinse with sterile water for more than 5 times for later use;

[0045]Seed imbibition and germination adopt the following steps: place the above-mentioned treated seeds in a sterile container covered with filter paper, disperse th...

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Abstract

The invention discloses a method for improving the germination rate and the survival rate of sophora alopecuroide seeds of wild medicinal plants and sophora alopecuroide seeds and belongs to the technical field of seed culture. The method comprises the following steps that: firstly 80-98% sulfuric acid is adopted to soak the seeds for 1-1.5 hours, then more than 95% alcohol is adopted to soak the seeds for 18-30 seconds, and then a sodium hypochlorite solution with the active chlorine content of being 3-6% is adopted to soak the seeds for 3-8 minutes; and the concentrations and the treating time of reagents among all the treating steps have influences on the germination rate, the germination uniformity and the survival rate of the seeds respectively. The method disclosed by the invention has the beneficial effects that firstly the treatment with concentrated sulfuric acid is carried out to break and remove tissue structures of horny layers of outer skin layers and palisade layers of middle skin layers of the seeds, due to coverage to seed holes of mature seeds, the structures have influences on water absorption and oxygen entering into the seeds; and next, the alcohol is used for eliminating polar substances in the skin layer corroded by the sulfuric acid, so that the sodium hypochlorite solution is easy to be used for fully soaking the seed coats in the next step and kill microorganisms on/in the skin layer.

Description

technical field [0001] The invention specifically relates to a method for improving the germination rate and survival rate of Sophora sophora seeds, and Sophora sophora seeds treated by the method, and belongs to the technical field of seed cultivation. Background technique [0002] Sophora alopecuroides L. is widely distributed in the desert areas of northwest my country and the saline-alkali habitats in northern my country. It is a medicinal plant of Sophora alopecuroides and has important value in medicine development and ecological maintenance. At present, the amount of wild resources of Sophora sophora in my country has dropped significantly. Although the seed setting rate of Sophora sophora per plant is very high, the pods are not easy to crack and the seeds are not easy to scatter under natural conditions, and the hard seed rate is extremely high, which is not conducive to the normal germination and survival of seeds. . In addition, the seed coat is polluted by mold, ...

Claims

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Application Information

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IPC IPC(8): A01C1/00
Inventor 赵龙飞徐亚军李彩虹
Owner SHANGQIU NORMAL UNIVERSITY
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