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Enrichment of low temperature-resistant heterotrophic simultaneous nitrification-denitrification microbial agent and application thereof in anoxic denitrification of sewage

A technology of heterotrophic nitrification and nitrifying bacteria agent, applied in biological water/sewage treatment, water/sludge/sewage treatment, anaerobic digestion treatment, etc., can solve the problem of low removal effect of ammonia nitrogen and nitrate nitrogen and low efficiency of functional flora , It is difficult to meet the emission standards and other problems, to achieve the effect of reducing the initial cost and dosage, stable denitrification effect and rapid growth

Active Publication Date: 2014-08-13
PEKING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in the cold winter, the efficiency of the functional flora in many sewage treatment plants is low, and the removal effect of ammonia nitrogen and nitrate nitrogen is low, resulting in high total nitrogen in the effluent, making it difficult to meet the discharge standards

Method used

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  • Enrichment of low temperature-resistant heterotrophic simultaneous nitrification-denitrification microbial agent and application thereof in anoxic denitrification of sewage
  • Enrichment of low temperature-resistant heterotrophic simultaneous nitrification-denitrification microbial agent and application thereof in anoxic denitrification of sewage
  • Enrichment of low temperature-resistant heterotrophic simultaneous nitrification-denitrification microbial agent and application thereof in anoxic denitrification of sewage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Implementation example 1: Enrichment of low temperature resistant heterotrophic nitrifying aerobic denitrifying bacteria agent

[0020] The aerobic seed sludge taken from a special reactor for treating synthetic sewage in a low-temperature laboratory is used for domestication and enrichment. Since the functional bacteria are heterotrophic bacteria, mixed wastewater containing organic carbon, ammonia nitrogen and nitrate nitrogen was used for enrichment in order to maximize the acquisition of heterotrophic simultaneous nitrifying and denitrifying bacteria. The entire enrichment process is divided into the following two steps:

[0021] (1) In a 250mL shake flask, enrichment was carried out with the mixed sewage of ammonia nitrogen and nitrate nitrogen containing organic carbon, and the rotation speed of the shaker was 150rpm to ensure that the bacterial agent and synthetic sewage were evenly mixed. At this stage, in order to ensure the rapid expansion of heterotrophic ni...

Embodiment 2

[0029] Implementation Example 2: Denitrification Ability of 250mL Shake Flask Aerobic Enrichment of Low-Temperature Bacteria under Anoxic Conditions

[0030] Take a certain amount of cold-resistant heterotrophic nitrification and aerobic denitrification bacterial agent enriched in the first stage and pour it into a 1L beaker, the initial MLSS is about 2500mg / L, and place it on a magnetic stirring device. Adding ammonia nitrogen and nitrate nitrogen mixed sewage containing organic carbon to determine its denitrification ability under low temperature conditions. The aeration rate was controlled at 0.1L / min, so that the initial DO was about 0.8-1.0mg / L. Sample 8mL every 1h in a 10mL centrifuge tube, and measure the DO, temperature and pH value in the system. The liquid sample was centrifuged at 8000rpm for 5min, and the supernatant was taken to measure NH 4 -N, NO 2 -N, NH 3 -N, DO and temperature. The pH in the system is controlled at 7.0-8.0.

[0031] Experimental results...

Embodiment 3

[0032] Implementation Example 3: Denitrification Ability of 5L SBR Anoxic Enrichment and Low Temperature Resistant Bacteria under Anoxic Conditions

[0033] In order to realize the rapid expansion of heterotrophic nitrification and aerobic denitrification sludge, the enrichment environment in stage 1 is aerobic. In order to improve the anoxic synchronous nitrification and denitrification ability of the low-temperature-resistant bacterial agent, the enrichment environment of the system was adjusted to anoxic in stage 2. After a period of anoxic enrichment, the denitrification capacity under low temperature and anoxic conditions was measured.

[0034] Take a certain amount of cold-resistant heterotrophic nitrification and aerobic denitrification bacteria enriched in the second stage and pour it into a 1L beaker, the initial MLSS is about 2500mg / L, and place it on a magnetic stirring device. Adding ammonia nitrogen and nitrate nitrogen mixed sewage containing organic carbon to d...

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Abstract

The invention relates to enrichment of a low temperature-resistant heterotrophic nitrification-aerobic / anoxic denitrification microbial agent and application thereof in wastewater treatment under an anoxic condition. The microbial agent is capable of realizing simultaneous nitrification and denitrification in an aerobic or anoxic environment, in the presence of both ammonia nitrogen and nitrate, with sodium acetate as a unique organic carbon source under a 10 DEG C low-temperature condition; therefore, inorganic nitrogen in wastewater is transformed into gaseous nitrogen and removal of total nitrogen is realized. The low temperature-resistant microbial agent thoroughly realizes maintenance and stabilization of activity of aerobic nitrification and heterotrophic denitrification bacteria under the low-temperature condition, and also has a very high total nitrogen removal rate under an anoxic condition. Besides, the heterotrophic microbial agent grows quickly and lays a good foundation for quick amplification and actual application. When applied to practical town sewage treatment, the microbial agent is capable of efficiently removing total nitrogen in the low-temperature environment under the single anoxic condition; and therefore, the problem that a traditional treating plant hardly reaches the national class A emission standard of total nitrogen in winter is solved and the utilization potentiality of the microbial agent is huge.

Description

technical field [0001] The invention relates to the enrichment of a low-temperature-resistant heterotrophic nitrifying aerobic / anoxic denitrifying bacterial agent and its application in wastewater treatment under anoxic conditions. The bacterial agent can not only grow and reproduce normally under low temperature conditions (10°C), but also show good total nitrogen removal effect through simultaneous nitrification and denitrification under anoxic conditions. Background technique [0002] The problem of eutrophication in my country's water bodies is prominent. Excessive discharge of nitrogen and phosphorus in sewage will aggravate the eutrophication of the receiving water body and the reduction of dissolved oxygen; the growth of some algae will release toxins, aggravate the deterioration of water quality, and lead to the death of a large number of aquatic organisms. There have been many studies on nitrogen and phosphorus removal in urban sewage, but the problem of efficient ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/28C02F3/34
Inventor 倪晋仁邹仕强姚硕
Owner PEKING UNIV
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