Method for refining dihydroquercetin from larch

Abstract

The invention discloses a method for refining dihydroquercetin from larch by using micro-porous resin. The method comprises the following steps: (1) after crushing the raw material larch wood, adding ethanol water solution to extract, collecting extracting solution and concentrating to obtain extract; (2) performing liquid phase adsorption separation on the extract by using micro-porous resin MCI, performing gradient elution by taking the ethanol water solution with volume fraction of 0-30 percent as an eluant, collecting and combining elution components with volume fraction of 20-25 percent, concentrating and drying to obtain a dihydroquercetin rough product; and (3) recrystallizing the dihydroquercetin rough product to obtain refined dihydroquercetin. According to the method, environment-friendly solvents such as water and ethanol are adopted, are pollution-free and can be recycled; the used adsorbent micro-porous resin introduces less impurities and can be reused for times; the obtained product has purity of greater than 99 percent and has no toxic ingredient residues, and the pharmaceutical-grade raw material can be directly obtained. The method has the advantages of simple route, economy, environmental friendliness and high yield and is suitable for industrial production.

Description

technical field

[0001] The invention relates to a method for refining dihydroquercetin from larch. Background technique

[0002] Dihydroquercetin (taxifolin, dihydroquercetin), also known as taxifolin, taxifolin, taxifolin, molecular formula C 15 h 12 o 7 , molecular weight: 304.25, structural formula: see formula I. It is a dihydroflavonol compound with the chemical name 5,7,3',4'-tetrahydroxydihydroflavonol. Dihydroquercetin widely exists in pinaceae plants, such as maritime pine, larch, and Douglas fir, and is the flavonoid compound with the largest content in larch, with a content of about 0.3-5.7%.

[0003]

[0004] (Formula I)

[0005] Dihydroquercetin has a strong antioxidant capacity, can effectively eliminate excess free radicals from the human body, and promote the permeability of capillaries. Its antioxidant properties are superior to many existing antioxidants, and there is no toxicity report, so it is widely used as an antioxidant additive in foods such...

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