Application of n-3 unsaturated fatty acid in preparation of medicine for preventing and treating traumatic brain injury
An unsaturated fatty acid, traumatic technology, applied in the field of medicine, can solve the problems of lack of treatment methods and preventive measures, and achieve the effects of improving learning and memory functions, facilitating recovery, and inhibiting white matter damage
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[0049] Embodiment 1 Animal experiment
[0050] 1. Experimental materials and methods
[0051] 1.1 Experimental animals and grouping
[0052] Clean grade juvenile male C57 / BL6 mice (provided by the Animal Center of the Chinese Academy of Sciences), 3 weeks old, 10-12g, the animals were divided into two groups before operation, one group was given a normal diet (excluding n-3PUFAs), that is, the RD group; One group was given a fish oil diet, that is, the FOD group, each 24g of food contained 1360mg of fish oil, and both groups were fed and intervened in the animal room of this laboratory for 60 days, at a room temperature of 20-22°C, with 12 hours of light and 12 hours of darkness every day, so that the animals could adapt to the environment . During the experiment, the mice were randomly divided into RD-sham group, FOD-sham group, RD-CCI group, and FOD-CCI group, and 40 mice in each group were evaluated for neurological function (12 mice) and water maze test (8 mice). Histol...
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