Candida selective culture medium for feces specimen and preparation method thereof

A technology for selecting culture medium and Candida, which is applied in the field of Candida selective culture medium for stool samples, which can solve the problems of low isolation rate of Candida and inability to grow Candida, and achieve the effect of reliable detection and high isolation rate

Inactive Publication Date: 2015-05-20
蒋欣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fungal selection medium currently used in clinical practice is mainly used for the isolation of Candida from clinical specimens such as urine, sputum, blood, bile, and ascites, such as potato dextrose agar (PDA) medium, which has a low isolation rate for Candida in stool samples. , the reason is mostly that Escherichia coli in the feces grows faster than Candida on the current fungal culture medium, and soon covers the culture medium, and Candida cannot grow

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1, the formula for preparing 1000ml medium contains: 3g of malt extract powder; 20g of beef heart powder; 1.2g of magnesium sulfate; 15g of agar; 0.02g of gentamicin sulfate; g; add distilled water to 1000ml. Preparation method: Dissolve malt extract powder, beef heart powder, magnesium sulfate, agar, aniline blue, calcium citrate tetrahydrate, and gentamicin sulfate in distilled water, mix well, distill water to 1000ml, 121°C, extinguish for 15 minutes Pack after sterilization.

Embodiment 2

[0023] Example 2, the formula for preparing 1000ml of medium contains: 10g of malt extract powder; 10g of beef heart powder; 2g of magnesium sulfate; 20g of agar; 0.005g of gentamicin sulfate; 0.4g of aniline blue; ; Add distilled water to 1000ml. Preparation method: Dissolve malt extract powder, beef heart powder, magnesium sulfate, agar, aniline blue, calcium citrate tetrahydrate, and gentamicin sulfate in distilled water, mix well, distill water to 1000ml, 121°C, extinguish for 15 minutes Pack after sterilization.

Embodiment 3

[0024] Example 3, the formula for preparing 1000ml medium contains: 3g of malt extract powder; 20g of beef heart powder; 1.2g of magnesium sulfate; 15g of agar; 0.02g of gentamicin sulfate; g, allithiamine 0.05g, glycocholic acid 0.1g; add distilled water to 1000ml. Preparation method: Dissolve malt extract powder, beef heart powder, magnesium sulfate, agar, aniline blue, calcium citrate tetrahydrate, gentamicin sulfate, allithiamine, glycocholic acid in distilled water, mix well, and distilled water Dilute to 1000ml, sterilize at 121°C for 15 minutes, and dispense.

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PUM

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Abstract

The invention discloses a candida selective culture medium for a feces specimen and a preparation method thereof. The invention is characterized in that every 1000ml of culture medium contains malt extract powder, beef heart powder, magnesium sulfate, agar, gentamicin sulphate, aniline blue, calcium citrate tetrahydrate and the balance of distilled water. The preparation method comprises the following steps: dissolving the malt extract powder, beef heart powder, magnesium sulfate, agar, aniline blue, calcium citrate tetrahydrate, gentamicin sulphate, allithiamine and glycocholic acid in an orchis saina turcz decoction, evenly mixing, diluting to the specified volume, sterilizing and subpackaging. Compared with the prior art, the invention has the characteristics of high detection reliability and high candida separation rate.

Description

technical field [0001] The parent case of the present invention is the name of the invention: a Candida selective culture medium for feces specimens and its preparation method, application number 2012100564838, application date: March 6, 2012, involving the field of microbiological culture, specifically It concerns a Candida selective medium for use in stool specimens. Background technique [0002] Microbial culture is a technology that uses artificial methods to grow and reproduce microorganisms, and selective medium is a medium designed to promote or inhibit certain types of organisms (such as cells or bacteria, etc.), using this medium can Desired microorganisms are separated from confounding microorganisms. Candida is the most common opportunistic pathogen among fungi, also known as Candida, which often parasitizes on human skin, oral cavity, vagina and intestinal mucosa, etc. , prone to candidiasis. At present, the clinical detection method of Candida is mainly Gram ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04C12R1/72
Inventor 蒋欣鲁莉殷凡于海涛
Owner 蒋欣
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