Candida selective culture medium for feces specimen and preparation method thereof

Abstract

The invention discloses a candida selective culture medium for a feces specimen and a preparation method thereof. The invention is characterized in that every 1000ml of culture medium contains malt extract powder, beef heart powder, magnesium sulfate, agar, gentamicin sulphate, aniline blue, calcium citrate tetrahydrate and the balance of distilled water. The preparation method comprises the following steps: dissolving the malt extract powder, beef heart powder, magnesium sulfate, agar, aniline blue, calcium citrate tetrahydrate, gentamicin sulphate, allithiamine and glycocholic acid in an orchis saina turcz decoction, evenly mixing, diluting to the specified volume, sterilizing and subpackaging. Compared with the prior art, the invention has the characteristics of high detection reliability and high candida separation rate.

Description

technical field [0001] The parent case of the present invention is the name of the invention: a Candida selective culture medium for feces specimens and its preparation method, application number 2012100564838, application date: March 6, 2012, involving the field of microbiological culture, specifically It concerns a Candida selective medium for use in stool specimens. Background technique [0002] Microbial culture is a technology that uses artificial methods to grow and reproduce microorganisms, and selective medium is a medium designed to promote or inhibit certain types of organisms (such as cells or bacteria, etc.), using this medium can Desired microorganisms are separated from confounding microorganisms. Candida is the most common opportunistic pathogen among fungi, also known as Candida, which often parasitizes on human skin, oral cavity, vagina and intestinal mucosa, etc. , prone to candidiasis. At present, the clinical detection method of Candida is mainly Gram ...

AI Technical Summary

Problems solved by technology

However, the fungal selection medium currently used in clinical practice is mainly used for the isolation of Candida from clinical specimens such as urine, sputum, blood, bile, and ascites, such as potato dextrose agar (PDA) medium, which has a low isolation rate for Candida in stool samples. , the reason is mostly that Escherichia coli in the feces grows faster than Candida on the current fungal culture medium, and soon covers the culture medium, and Candida cannot grow