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Hybrid antimicrobial peptide and preparation method thereof

A technology of hybrid antibacterial peptides and crude peptides, which is applied in the field of hybrid antibacterial peptides and their preparation, can solve problems such as limiting clinical applications, and achieve the effect of rapid bactericidal ability

Inactive Publication Date: 2013-11-27
GUANGZHOU GLAM BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Melittin has a strong inhibitory effect on both Gram-positive and negative bacteria, and has a strong hemolytic activity, which limits its clinical application

Method used

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  • Hybrid antimicrobial peptide and preparation method thereof
  • Hybrid antimicrobial peptide and preparation method thereof

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Embodiment Construction

[0031] The preferred embodiments of the present invention are given below in conjunction with the accompanying drawings to describe the technical solution of the present invention in detail.

[0032] According to the core functional sequence of catfish antimicrobial peptide (Parasin I) and glycine-rich melittin (Melittin), the invention designs a novel hybrid Antimicrobial peptide PAME, the N-terminal of the antimicrobial peptide molecule forms an amphipathic α-helical structure, the basic amino acid at the C-terminal makes the antimicrobial peptide molecule positively charged, and can bind to the negatively charged cell membrane, and the hybrid antimicrobial peptide abandons melittin The hemolytic activity, its antibacterial activity and stability are superior to single antimicrobial peptide molecule. The amino acid sequence of the hybrid antibacterial peptide of the present invention is KGRGKQGGKVRAKAKTRVLKVLTTG, and the hybrid antibacterial peptide of the present invention ...

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Abstract

The invention discloses hybrid antimicrobial peptide and a preparation method thereof. An amino acid sequence of the hybrid antimicrobial peptide is represented as KGRGKQGGKVRAKAKTRVLKVLTTG. The preparation method of the hybrid antimicrobial peptide includes the steps of material taking, reaction, purification, mass spectrometry analysis and identification and the like. The hybrid antimicrobial peptide, as well as the preparation method thereof, is relatively high in antibacterial activity and is safe and non-toxic.

Description

technical field [0001] The invention relates to the field of polypeptide synthesis, in particular to a hybrid antibacterial peptide and a preparation method thereof. Background technique [0002] The widespread use of antibiotics and the abuse of antibiotics lead to the resistance of bacteria to antibiotics. Therefore, finding and developing new antibiotics is a hot topic. Cationic antimicrobial peptides exist in a variety of organisms, and have broad-spectrum effects against Gram-positive and Gram-negative bacteria, fungi, and protozoa in the host's natural immunity. This type of antibacterial polypeptide contains more basic amino acids (positively charged under physiological conditions) and more hydrophobic amino acids. Due to the unique antibacterial mechanism, bacteria are not easy to develop drug resistance to it, and it is very promising as a clinical application. Useful antibiotics. At present, nearly 10 cationic antimicrobial peptide analogues have entered clinical...

Claims

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Application Information

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IPC IPC(8): C07K19/00C07K1/06C07K1/04
Inventor 何宏轩李本涛印遇龙夏新界王从峰李爱科佘锐萍李刚李帅伟
Owner GUANGZHOU GLAM BIOTECH
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