Tremella liquid cultivar culture method and special culture medium for cultivar

The technology of a liquid cultivar and a cultivation method is applied in the field of tremella liquid cultivar cultivation method and cultivar-specific medium, which can solve the problems of spit out black water in the inoculation hole, season delay, rotten ears, etc., achieve rapid germination and solve the problem of making a difficult effect

Active Publication Date: 2014-01-08
CHONGQING ACAD OF CHINESE MATERIA MEDICA
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AI Technical Summary

Problems solved by technology

At the end of spring, the time for seeding is delayed, and the season of mushroom cultivation is delayed. Often, when the primordium differentiates into fruiting bodies, the temperature is too high, and when the temperature exceeds 25°C, people will spit out black water from the inoculation hole, resulting in rotten ears. In late autumn, Because the seed supply is not timely, after the temperature drops, white mucus will be secreted from the acupoints after inoculation, which will lead to poor differentiation of primordium and affect the growth and development of fruiting bodies.

Method used

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  • Tremella liquid cultivar culture method and special culture medium for cultivar
  • Tremella liquid cultivar culture method and special culture medium for cultivar

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Embodiment 1

[0017] The investigation of embodiment 1 white fungus liquid cultivar cultivation method

[0018] 1. Strain material: Yinke No. 1

[0019] Liquid medium formula: 200g potatoes, 20g glucose, 1L water, 100ml / 250ml triangular flask;

[0020] Culture medium formula: sawdust, wheat bran, sucrose, gypsum powder, ratio of material to water, pH5.8-6.2;

[0021] Culture bag culture material formula: cottonseed hulls, bran, gypsum powder, magnesium sulfate, potassium dihydrogen phosphate;

[0022] 2. Cultivation steps of cultivars

[0023] 1. Liquid seed preparation: After the liquid medium is prepared, it is sterilized at 121°C for 30 minutes. Insert 4 pieces of white fungus (1×2cm) into each bottle, culture at 200rmp / min, 25°C for 5-10 days, after 3 days of white fungus culture, take a shovel of white fungus into the new medium, and cultivate under the same conditions for 3- 5 days;

[0024] 2. Preparation of cultivars: Stir the above raw and auxiliary materials evenly according ...

Embodiment 2

[0037] The influence of the cultivar medium formula of embodiment 2 different carbon-nitrogen ratios on tremella cultivation

[0038] In this embodiment, the wood chips used are oak wood chips with a carbon content of 50.2% and a nitrogen content of 1.10%; bran with a carbon content of 44.7% and a carbon content of 3.3%, and sucrose with a carbon content of 42.1%.

[0039] Total carbon content = sawdust weight × 0.502+ bran weight × 0.447+ sucrose × 0.421

[0040] Total nitrogen = sawdust weight × 0.011 + bran weight × 0.033

[0041] According to the carbon-nitrogen ratio of ①27:1, ②28:1, ③29:1, ④30:1, ⑤31:1, ⑥32:1 and other groups of carbon-nitrogen ratios to prepare tremella cultivar medium. Among them, the test formulas of sawdust, bran, sucrose and gypsum powder are: ①group 66:32:2:2, ②group 69:29:2:2, ③group 71:27:2:2, ④group 73:25:2:2, ⑤ group 75:23:2:2, ⑥ group 77:21:2:2.

[0042] Bottled as usual. The water content is 60%. 10 bottles were made for each of the 6 gr...

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Abstract

The invention provides a tremella liquid cultivar culture method and a special culture medium for a cultivar. The tremella liquid cultivar culture method includes the following steps that firstly, bevel culture media are used for culturing Hypoxylon sp and tremella bacteria respectively; secondly, strains of the Hypoxylon sp and strains of the tremella bacteria are moved into the liquid culture media for culture respectively; thirdly, the liquid strains of the Hypoxylon sp are mixed into the liquid strains of the tremella bacteria according to the volume percent of 1-5% and inoculated into a culture bottle for culture, and the cultivar is obtained through preparation. According to the tremella liquid cultivar culture method, the problem that a traditional cultivar is difficult to prepare is solved, meanwhile, the liquid strains are faster in mycelia running, matched growth of the liquid strains and symbiotic bacteria can be effectively achieved, and the yield of tremella produced by the liquid cultivar prepared with the method is improved by more than 20% compared with the yield of tremella produced by the traditional cultivar.

Description

technical field [0001] The invention relates to a method for cultivating fungi, in particular to a method for cultivating tremella liquid cultivars and a special culture medium for cultivars. technical background [0002] Tremella fuciformis Beyk, also known as Tremella fuciformis Beyk, is a traditional edible and medicinal fungus in my country. After Tremella substitute cultivation, the production cycle is short, only 35-40 days from inoculation to harvest. Tremella fungus strains suitable for substitute cultivation generally have a short validity period of about 15 days. The quality of cultivated species directly affects the cultivation quality of Tremella. Therefore tremella seed production technology is the most difficult kind in many edible fungi. Tremella is a mesophilic strain. Under natural climate conditions, the cultivation season is mainly concentrated in spring and autumn, and the peak period of strain use is relatively concentrated. Since the 17-20 days of cul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 贺元川贺忠毅鲁增辉陈仕江杨勇谭红军
Owner CHONGQING ACAD OF CHINESE MATERIA MEDICA
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