Tremella liquid cultivar culture method and special culture medium for cultivar
The technology of a liquid cultivar and a cultivation method is applied in the field of tremella liquid cultivar cultivation method and cultivar-specific medium, which can solve the problems of spit out black water in the inoculation hole, season delay, rotten ears, etc., achieve rapid germination and solve the problem of making a difficult effect
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Embodiment 1
[0017] The investigation of embodiment 1 white fungus liquid cultivar cultivation method
[0018] 1. Strain material: Yinke No. 1
[0019] Liquid medium formula: 200g potatoes, 20g glucose, 1L water, 100ml / 250ml triangular flask;
[0020] Culture medium formula: sawdust, wheat bran, sucrose, gypsum powder, ratio of material to water, pH5.8-6.2;
[0021] Culture bag culture material formula: cottonseed hulls, bran, gypsum powder, magnesium sulfate, potassium dihydrogen phosphate;
[0022] 2. Cultivation steps of cultivars
[0023] 1. Liquid seed preparation: After the liquid medium is prepared, it is sterilized at 121°C for 30 minutes. Insert 4 pieces of white fungus (1×2cm) into each bottle, culture at 200rmp / min, 25°C for 5-10 days, after 3 days of white fungus culture, take a shovel of white fungus into the new medium, and cultivate under the same conditions for 3- 5 days;
[0024] 2. Preparation of cultivars: Stir the above raw and auxiliary materials evenly according ...
Embodiment 2
[0037] The influence of the cultivar medium formula of embodiment 2 different carbon-nitrogen ratios on tremella cultivation
[0038] In this embodiment, the wood chips used are oak wood chips with a carbon content of 50.2% and a nitrogen content of 1.10%; bran with a carbon content of 44.7% and a carbon content of 3.3%, and sucrose with a carbon content of 42.1%.
[0039] Total carbon content = sawdust weight × 0.502+ bran weight × 0.447+ sucrose × 0.421
[0040] Total nitrogen = sawdust weight × 0.011 + bran weight × 0.033
[0041] According to the carbon-nitrogen ratio of ①27:1, ②28:1, ③29:1, ④30:1, ⑤31:1, ⑥32:1 and other groups of carbon-nitrogen ratios to prepare tremella cultivar medium. Among them, the test formulas of sawdust, bran, sucrose and gypsum powder are: ①group 66:32:2:2, ②group 69:29:2:2, ③group 71:27:2:2, ④group 73:25:2:2, ⑤ group 75:23:2:2, ⑥ group 77:21:2:2.
[0042] Bottled as usual. The water content is 60%. 10 bottles were made for each of the 6 gr...
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