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Non-specific reaction inhibitor, and method and kit for inhibiting non-specific reaction

A non-specific reaction and inhibitor technology, applied in the field of non-specific reaction inhibitors, can solve problems such as insufficient effect and unsatisfactory clinical examination site, and achieve good results

Active Publication Date: 2014-03-05
PHC CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, although these methods are effective in suppressing non-specific reactions to a certain extent, their effects are insufficient in some samples, and they are not satisfactory in the field of clinical examination where misdiagnosis may cause serious consequences.

Method used

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  • Non-specific reaction inhibitor, and method and kit for inhibiting non-specific reaction
  • Non-specific reaction inhibitor, and method and kit for inhibiting non-specific reaction
  • Non-specific reaction inhibitor, and method and kit for inhibiting non-specific reaction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] "Example 1: Production of Non-Specific Reaction Inhibitor (SMCC-BSA)"

[0074] Bovine serum albumin (BSA: Sigma) was dissolved at 10 mg / mL in 0.1 mol / L HEPES (pH 7.0), followed by 50 mg / mL Sulfo-SMCC dissolved in dimethyl sulfoxide (DMSO). (4-(N-maleimidomethyl)cyclohexane-1-carboxylate sulfosuccinimide) (PIAS) solutions were mixed and left to stand at 37°C overnight. A 114 mg / mL cysteamine HCl solution was added to this reaction solution to block the terminal maleimide groups. It was used in the following studies as a non-specific reaction inhibitor.

[0075] [Chem 2]

[0076]

Embodiment 2

[0077] "Example 2: Construction of HBs antigen assay system"

[0078] (1) Preparation of magnetic particle-bound antibody solution

[0079] (1-1) Magnetic particle-bound antibody solution produced by the maleimide method

[0080] After binding magnetic particles (JSR) and BSA (Sigma), a magnetic particle solution was prepared by binding anti-HBs mouse monoclonal antibody using Sulfo-SMCC (PIAS). In addition, the anti-HBs mouse monoclonal antibody was prepared by producing recombinant HBs antigen using CH1 cells and immunizing mice with the recombinant HBs antigen according to a known method.

[0081] (1-2) Magnetic particle-bound antibody solution produced by EDC method

[0082] A magnetic particle solution obtained by binding magnetic particles (JSR) and anti-HBs mouse monoclonal antibody with carbodiimide (Sigma) was prepared.

[0083] (2) Production of labeled antibody solution

[0084] The anti-HBs rabbit polyclonal antibody was labeled with alkaline phosphatase (ALP) ...

Embodiment 3

[0090] "Example 3: Confirmation of the effect of avoiding the influence of interfering substances in samples by non-specific reaction inhibitors"

[0091] In the method of Example 2, the non-specific reaction inhibitor produced in Example 1 was added at a final concentration of 0.03%, and the presence or absence of its effect was examined.

[0092] One case of HBs antigen-positive samples obtained from the HBs antigen test-dependent patient group and five cases of HBs antigen-negative samples in which non-specific reactions were confirmed were measured under each condition. In the case of using a magnetic particle-bound antibody sensitized by the maleimide method, stronger nonspecific Heterotropic reaction, but this nonspecific reaction was significantly suppressed after adding a nonspecific reaction inhibitor to the maleimide-sensitized magnetic particle-bound antibody.

[0093] The above suggests that the non-specific samples used in the study contained non-specifically rea...

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Abstract

Provided is a non-specific reaction inhibitor for inhibiting the occurrence of any non-specific reaction associated with a measurement procedure in a simple and effective manner to achieve the accurate detection and quantification of a trace component (a target substance) in a sample in an immunoassay. The non-specific reaction inhibitor comprises a substance represented by formula (I) [wherein R1 and R2 together form a double bond between carbons to which R1 and R2 are respectively bound directly, or R1 represents a hydrogen atom and R2 represents a group formed by removing H from an SH-group-containing compound; B represents a carrier; and L represents a spacer arm moiety].

Description

technical field [0001] The present invention relates to a non-specific reaction inhibitor, a method for suppressing a non-specific reaction using the inhibitor, and an immunoassay kit comprising the inhibitor, which is used in an immunological assay for suppressing a non-specific reaction, the non-specific reaction The heterosexual reaction is an obstacle to the accurate detection and quantification of trace amounts of target substances. Background technique [0002] Immunological assays using antigen-antibody reactions are widely used in clinical examinations because they can specifically detect or measure trace components to be measured with good precision. It has been recognized that in these immunological assays, the reliability of measured values ​​is impaired due to non-specific reactions other than specific antigen-antibody reactions, which are originally intended target substances. This phenomenon is caused by the reaction of components other than the target substan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/543
CPCG01N33/5306C07D207/416
Inventor 冈村佳和田代茂
Owner PHC CORP
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