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A kind of Bacillus megaterium having lytic effect on Microcystis aeruginosa and its application

A technology of Bacillus megaterium and Microcystis aeruginosa, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve problems such as public health, livestock and water supply impacts, damage to water ecosystems, eutrophication pollution, etc.

Active Publication Date: 2016-04-13
LIAONING UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, more than 66% of lakes and reservoirs in my country are under eutrophication pollution to varying degrees, which not only destroys the balance of the water ecosystem, but also produces a large amount of algae toxins from the dominant Microcystis in the water where the algae bloom occurs. and other metabolites have negative impacts on public health, livestock and water supplies

Method used

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  • A kind of Bacillus megaterium having lytic effect on Microcystis aeruginosa and its application
  • A kind of Bacillus megaterium having lytic effect on Microcystis aeruginosa and its application
  • A kind of Bacillus megaterium having lytic effect on Microcystis aeruginosa and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Embodiment 1 dissolves the method for Microcystis aeruginosa

[0066] (1) Strain A 0 cultivation

[0067] Medium formula: distilled water 1000mL, beef extract 3g, peptone 10g, NaCl 5g, pH value 7.0~7.2.

[0068] Method: Put 50mL of prepared culture medium into nine 250mL Erlenmeyer flasks, sterilize at 121°C for 20min, cool at room temperature, inoculate strain A with 5% (v / v) inoculum 0 Put the seed liquid into each triangular flask, then put it into a constant temperature oscillator to vibrate, the rotation speed is 160r / min, the temperature is 30°C, take out a bottle every three hours, observe and count under the microscope with a hemocytometer, Calculate the bacterial density, measure the absorbance at 660nm wavelength at the same time, draw the growth curve with time as the abscissa, bacterial density and OD660 as the ordinate. see results image 3 .

[0069] from image 3 It can be seen that 0~3h is the lag phase of bacterial growth, 3~9h is the logarithmic ...

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Abstract

The invention relates to bacillus megaterium capable of dissolving microcystis aeruginosa and an application thereof. The bacterial strain A0 can be used for dissolving the microcystis aeruginosa. When the adding amount is 7.5%, the dissolving function is very obvious in the 6th day of cocultivation, the amount of frustule is 66.7% lower than the matched group and the content of frustule chlorophyll alpha is 35.2% lower than the matched group in the 6th day.

Description

technical field [0001] The invention relates to a bacterial strain screened from eutrophic water body with algalytic effect. The bacterial strain can dissolve the main alga causing eutrophication of the water body—Microcystisaeruginosa. [0002] A kind of bacillus megaterium that the present invention claims has dissolving action to Microcystis aeruginosa is named after: bacillus megaterium (Bacillus megaterium) A 0 , has been preserved in the China Center for Type Culture Collection on September 27, 2013, the address is: Wuhan University, Wuhan, China, and the number is CCTCCNO: M2013452. Background technique [0003] In recent years, with the improvement of industrialization, the acceleration of urbanization and the continuous increase of population, human activities have become more frequent and profoundly affecting the water environment. The accumulation of nitrogen and phosphorus in the water body reaches a certain amount, which will cause eutrophication of the water b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/11
Inventor 付保荣郭鑫杨玉东张润洁付豪苗斌
Owner LIAONING UNIVERSITY
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