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Culture mediums for tissue culturing of maiden pink

A technique of tissue culture and girl dianthus, applied in the field of plant biology, can solve the problems of less seedlings, degeneration, quality of disease infection, etc.

Active Publication Date: 2014-04-16
INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The artificial propagation of dianthus still mainly adopts seeding method and cutting method, the number of reproduction is limited, the time is long, and it is easy to cause adventitious bud glass flower, disease infection and quality degradation
At present, there are few studies on tissue culture propagation that can be used to solve the problems of shortening seedling cultivation time, industrial production, vitrification of seedlings, and renewal and rejuvenation. Therefore, it has become one of the urgent tasks for scientific researchers to carry out in-depth research on tissue culture of dianthus.

Method used

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  • Culture mediums for tissue culturing of maiden pink
  • Culture mediums for tissue culturing of maiden pink
  • Culture mediums for tissue culturing of maiden pink

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1. The material and screening statistical method of girl carnation explant

[0018] 1. Materials

[0019] The dianthus used in the experiment was collected from the experimental nursery of the Drought Resistant Plant Research Institute of Hexinyuan Mengcao Drought Resistant Greening Co., Ltd. in Inner Mongolia. According to the progress of the experiment, samples were taken at different times and supplemented at any time. Harvest the stem tips of young dianthus shoots about 0.5-1.0cm long, wash them with running water, soak them in 70% alcohol for 10 seconds, then disinfect them with 0.1% mercury liter for 5 minutes, rinse them twice with sterile water, 5 minutes each time , inoculated on the improved primary medium (MS) to establish a clone of test-tube plantlets. Select the clustered buds of about 10 days of subculture, cut off the base of the clustered buds; take the shoot tips of the clustered buds, about 0.5-1.0 cm in length, as explants for regeneratio...

Embodiment 2

[0025] Example 2. Screening of optimal medium for each stage of girl carnation tissue culture

[0026] Using MS as the basic medium, using 3.5g / L carrageenan as the solidifying agent, 3% edible sugar instead of sucrose as the carbon source, tap water instead of distilled water, and adding different ratios of cytokinin and auxin to the culture medium filter.

[0027] 1. Selection of induction differentiation medium

[0028] Set the concentration of 6-BA to five concentration gradients of 0.01mg / L, 0.05mg / L, 0.1mg / L, 0.2mg / L, and 0.5mg / L, and set the concentration of NAA to 0.01mg / L, 0.05mg / L, 0.1mg / L, 0.2mg / L four concentration gradients, carry out different combinations, each bottle is inoculated with 2-3 young girl carnation stem tips (the stem tip length is about 0.5-1cm), and observe the shoot tips on different media The occurrence of adventitious buds.

[0029] It can be seen from Table 1 that seven kinds of hormone combinations can induce dianthus to differentiate into...

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Abstract

The invention relates to culture mediums for tissue culturing of a maiden pink. The optimal culture mediums for different stages in the tissue culturing of the maiden pink are screened; and the matching proportions of various hormones in the culture mediums are determined, so that the culture mediums prepared according to a formula provided by the invention can meet the nutritional requirement and the growing development requirement of the maiden pink in all stages. The culture media respectively comprise: an initial induction culture medium formed by MS, 0.01mg / L of 6-BA and 0.05mg / L of NAA (Naphthalene Acetic Acid), a mulitiplication culture medium formed by the MS, 0.01mg / L of 6-BA and 0.05mg / L of NAA, and a rooting culture medium formed by 1 / 2 of MS and 0.05mg / L of IBA (Indole Butyric Acid). The culture mediums are particularly suitable for the tissue culturing of the maiden pink.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a culture medium of various stages in the tissue culture of dianthus dianthus. The present invention screens out the optimal culture medium for different periods in the tissue culture of the dianthus dianthus, determines the ratio of each hormone in the medium, and the medium prepared according to the formula of the present invention can meet the nutritional needs and requirements of the different periods of the dianthus dianthus. growth and development. Background technique [0002] Dianthus is a perennial grass flower cultivated as one or two years. It is an important ornamental flower because of its rich color, long flowering period and extensive cultivation and management. There are more than 600 species in the world, widely distributed in the northern temperate zone, mainly in Eurasia, especially in the Mediterranean region, and a few in North America and Nort...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 马怀林王召明高秀梅田志来
Owner INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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