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A Pseudomonas strain that can degrade pva
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A Pseudomonas, PVA1799 technology, applied in the field of bioengineering, can solve environmental pollution and other problems
Active Publication Date: 2016-05-18
JIANGNAN UNIV
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The commonly used desizing method is to use hot water to wash off PVA on the surface of cotton fabrics, and sometimes use oxidants such as NaBrO 2 etc., which leads to a large amount of PVA and oxidants in the wastewater discharged from textile factories, which will cause great pollution to the environment
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Embodiment 1
[0014] Qualitative screening of embodiment 1 specific degradation PVA bacterial strain
[0015] 1. Source of strain: activated sludge
[0016] 2. Cultivation method: 10g of activated sludge obtained by sampling was added to 90mL of sterile normal saline, and shaken on a shaking table (200rpm) for 6h.
[0017] 3. Screening: After diluting the bacterial suspension to different times, spread it on a PVA sole carbon source plate, incubate at 30°C, and develop color by the Finley method, select the strains that can produce transparent circles and perform plate purification repeatedly for 3 times, and finally obtain the pure target strains , was deposited in China Center for Typical Microorganisms Collection on October 13, 2013, with the deposit number CCTCCNO: M2013468, and the deposit address is: Wuhan, Wuhan University, China.
Embodiment 2
[0018] The mensuration of embodiment 2PVA degrading enzyme enzymatic activity
[0019] 1. Finley method (improved): Take 400 μL of fermentation broth supernatant, add it to a standard 10 mL colorimetric tube, add 3 mL of 25 g / L boric acid solution, add 300 μL of 0.1 mol / L I2-KI, and add water to 10 mL. React in the dark for 5 minutes, and measure the absorbance at a wavelength of 690 nm.
[0020] 2. Determination of the total enzyme activity of PVA-degrading enzymes: take about 10mL of fermentation broth, and break it by ultrasonic wave at 4°C for about 15 minutes, which is the crude enzyme solution (including extracellular enzymes and intracellular enzymes), pass through a microporous membrane (pore size 0.45 μm, diameter 50mm), and then dialyzed with 0.1mol / L potassium phosphate buffer (pH7.5) for 24h to obtain the crude enzyme solution. Add 0.5mL crude enzyme solution and 0.5mL substrate (1g / LPVA1799 dissolved in pH 7.5 potassium phosphate buffer) to a 5×15 test tube, reac...
Embodiment 3
[0021] Degradation of 1g / LPVA1799 in solution by bacterial strain TD5 in embodiment 3
[0022] 1. Preparation of seeds
[0023] Take 100 μL of the TD5 strain preserved in a glycerol tube at -80°C and inoculate it into the seed medium according to claim 3, and culture it at 30°C for 16 hours.
[0024] 2. TD5 degrades PVA1799 in the fermentation medium
[0025] Get the 3OD seed culture medium to centrifuge and retain the thalline, and resuspend the seed thalline with the fermentation medium to inoculate into the fermentation medium described in claim 3. The results showed that PVA1799 in the fermentation medium could be completely degraded within 7 days.
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Abstract
The invention discloses a pseudomonas strain capable of degrading PVA (polyvinyl alcohol), which is named pseudomonas sp.TD5 and collected in the China Center for Type Culture Collection (CCTCC) on October 13, 2013, with a collection number CCTCC No:M2013468. The strain is screened from active sludge specifically through enrichment of microorganisms, separation of target strains and screening by a transparent zone process; the enzyme activity of the PVA degrading enzyme can be detected by the Finley method, and the PVA1799 degrading effect of the strain can be verified. The strain can efficiently degrade the PVA1799 pollutant components in a culture medium and desizing sewage and can effectively eliminate the pollutant PVA1799, thereby reducing the environmental pollution and lowering the sewage treatment cost.
Description
Technical field: [0001] The invention relates to a Pseudomonas bacterial strain degrading PVA1799 and its application, in particular to a Pseudomonas bacterial strain capable of independently degrading PVA1799 in an environment, belonging to the technical field of bioengineering. Background technique: [0002] Polyvinyl alcohol (PVA) is widely used as a sizing agent in the textile industry, among which PVA1799 occupies a considerable proportion. Cotton fabrics sized with PVA must be desized to increase the water absorption of cotton fabrics. The commonly used desizing method is to use hot water to wash off PVA on the surface of cotton fabrics, and sometimes use oxidants such as NaBrO 2 etc., which leads to a large amount of PVA and oxidants in the wastewater discharged from textile mills, which will cause great pollution to the environment. [0003] If PVA-degrading enzymes or PVA-degrading bacteria can be used in the desizing process of the textile industry, the biodegrad...
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