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Capture of breast cancer related genes, and preparation method and application of probes

A technology for gene capture and capture probes, which is applied in the field of detection of nucleotide compositions related to cancer, can solve problems such as increasing costs, and achieve the effects of solving use efficiency, ensuring consistent capture, and efficient early detection

Inactive Publication Date: 2014-04-30
SHANGHAI MAJORBIO BIO PHARM TECH
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Problems solved by technology

But this also increases the cost. For some less economically developed areas, the combined use of these detection methods has great limitations.

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  • Capture of breast cancer related genes, and preparation method and application of probes
  • Capture of breast cancer related genes, and preparation method and application of probes

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Embodiment Construction

[0029] In order to make the purpose, technical solution and advantages of the present invention clearer, various embodiments of the present invention are described in detail below. However, those of ordinary skill in the art can understand that, in each implementation manner of the present invention, many technical details are provided for readers to better understand the present application. However, even without these technical details and various changes and modifications based on the following implementation modes, the technical solution claimed in each claim of the present application can be realized.

[0030] Specifically, the present invention constructs a capture probe library for the specific detection of breast cancer based on the existing or obtained information on breast cancer high-risk pathogenic genes; the target genomic region DNA template to be captured is contained in the test sample Fragmentation, adding adapters (joints) at both ends, and enriching for dete...

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Abstract

A capture method of breast cancer related genes is provided. Probes obtained according to a construction method of a breast cancer related gene capture probe database are subjected to hybridization with a to-be-captured target genome region-containing DNA template in a to-be-detected sample. Fragments obtained by capture are subjected to high throughput sequencing; and then based on known information of breast cancer pathogenic genes, data analysis is carried out by utilizing a bioinformatics method, and thus heritable variation conditions of nucleic acid sequences in the to-be-detected sample are obtained. The provided various methods and the obtained database (or clone database) can adjust the relative capture efficiency so as to ensure consistency capture on all the target genes, and the problem that the use efficiency of an NGS sequencing platform is greatly hindered because of heterogeneity and high cost of chip capture reported in current literatures is solved.

Description

technical field [0001] The present invention relates to a nucleotide composition for detecting cancer-related, in particular to a method based on the preparation of capture probe sets (specifically hybridized target gene fragments) to achieve selective capture and amplification of specific exons or target genomic regions. Background technique [0002] Breast cancer is caused by gene mutations in breast epithelial cells under the action of various carcinogenic factors, resulting in uncontrolled cell proliferation. According to statistics, there were 209,060 new cases of breast cancer diagnosed in the United States in 2010, and 40,230 patients died of breast cancer. [0003] According to statistics, in recent years, while the incidence of breast cancer has continued to rise, the mortality rate of breast cancer in developed countries such as Europe and the United States has shown a downward trend. Extensive breast cancer screening and increasing early detection rate are consi...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C40B50/06C40B40/02C12Q1/68C12N15/11C12N15/66
Inventor 张一桐张祥林胡秋萍陈昌岳陶晔
Owner SHANGHAI MAJORBIO BIO PHARM TECH
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