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Nannochloropsis sp. mutant strain, and applications thereof

A technology of Nannochloropsis and mutant strains, applied in the field of microbial engineering, to achieve the effects of improved resistance to diseases and insect pests, good breeding conditions, and good environmental adaptability

Active Publication Date: 2014-05-07
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Patent application CN102492626 discloses a Nannochloropsis sp. algal strain ENN1003A, which has the advantages of fast growth, high culture density, high oil content, and rich in long-chain unsaturated fatty acids such as EPA and protein , suitable for the production of biodiesel and the development of health products, medicines, and food, but there is still room for improvement in the performance of this algae strain

Method used

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  • Nannochloropsis sp. mutant strain, and applications thereof
  • Nannochloropsis sp. mutant strain, and applications thereof
  • Nannochloropsis sp. mutant strain, and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Example 1 Mutation screening, indoor cultivation and oil accumulation of mutant strain ENN11-2

[0022] The fresh sterile algae liquid of Nannochloropsis sp. wild strain ENN1003A in the logarithmic phase was subjected to EMS mutagenesis treatment, and the initial concentration of mutagenesis treatment was 6.3×10 6 / ml, add 1% (by volume) EMS to the algae liquid, place at 37°C and incubate for 60min with shaking in the dark. With 10% (by mass) sterile NaS 2 o 3 The solution was terminated, washed 3 times with seawater medium, and after 33 hours of dark culture, the light culture was taken out until the lethality was stable, and the algae liquid was plated on the f / 2 solid plate with a DCMU concentration of 1.5uM at 300 / plate, and individuals were picked. A total of 30 larger single algae colonies were screened in the Erlenmeyer flask, and ENN11-2 with a fast growth rate was selected for evaluation.

[0023] Inoculate the ENN11-2 green swimming cells in the logarithm...

Embodiment 2

[0038] Outdoor cultivation and oil accumulation of embodiment 2 mutant strain ENN11-2

[0039] Inoculate the algae species in the logarithmic growth phase in a 50*50*5cm plate reactor, use seawater medium for cultivation, and place them outdoors under natural conditions for growth and cultivation. The natural conditions during the cultivation period are as follows: the cultivation period is 11 days, The temperature range from 9:00 to 17:00 is mainly between 25-37°C, and occasionally the highest temperature reaches 40°C. From 9:00 to 17:00, the range of light changes is mainly 200-700umol / m on the sunny side 2 Between / s, occasionally the light intensity reaches 800umol / m 2 / s. A certain percentage of CO is injected during the day 2 (more than 95% purity), the pH value is controlled between 7-9. Sampling was taken intermittently during the cultivation process to determine the dry weight (results in image 3 ). After the cultivation, the algae liquid is collected, and th...

Embodiment 3

[0042] Example 3 Mutant strain ENN11-2 contains high CO 2 waste gas farming

[0043] Inoculate the algal species in the logarithmic growth phase in a 50*50*13cm plate reactor, use seawater medium for cultivation (without adding C source), and place them outdoors for growth and cultivation under natural conditions. The natural conditions during the cultivation period are as follows: During the experiment, the temperature reached 39°C and above for only 7 days. The rest of the time is mostly around 30°C, and the temperature drops to 20-30°C in the later stage. During the experiment, the amount of visible light on both sides reached 8000 μmol / m in 8 hours 2 / s(6.25MJ / m 2 ) and above for 14 days, 7000-8000μmol / m 2 / s has 4 days; 6600μmol / m 2 There are 10 days below / s and 3 days of rainy days. The weather conditions are average, and the number of days with better light accounts for 58% of the experimental days. A certain percentage of CO is injected during the day 2 ...

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Abstract

The invention discloses a nannochloropsis sp. mutant strain ENN11-2 with a preservation number of CGMCC No.6009, and applications thereof. The nannochloropsis sp. mutant strain ENN11-2 can be used for preparing medicines or health products which are used for preventing and treating cardiovascular and cerebrovascular diseases; for producing or preparing food; for producing baits or animal feed; for producing biofuel and especially biodiesel; for treatment of sewage containing nitrogen and phosphor; for emission reduction of CO2; and for producing long-chain unsaturated fatty acid and especially EPA. Compared with wild strain, growth speed of the nannochloropsis sp. mutant strain ENN11-2 is increased 10%, EPA content is increased 10%, performance is relatively stable, environmental suitability is excellent, disease resistance and insect pest resistance are high, and outdoor validation of the nannochloropsis sp. mutant strain ENN11-2 is realized.

Description

technical field [0001] The invention relates to the field of microbial engineering, in particular to Nannochloropsis sp. and its application. Background technique [0002] Extensive use of fossil energy has greatly exacerbated atmospheric pollution and climate change, as well as the increase in the area of ​​acid rain. It is imperative to find new sustainable and environmentally safe biological resources to produce biofuels to replace fossil fuels. Biodiesel is an environmentally friendly biofuel prepared from unprocessed or used vegetable oils and animal fats through different chemical reactions. This biofuel can be used like diesel. Biodiesel is a proven fuel, which can partially replace fossil diesel in terms of energy properties, and is one of the most promising liquid biofuels. [0003] Microalgae are a class of low-level plants that grow in water with a wide variety and are extremely widely distributed. It is a cell factory driven by sunlight. It absorbs CO through e...

Claims

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Application Information

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IPC IPC(8): C12N1/12A61K36/05A61P9/00A23L1/29A23K1/14A01K97/04C12P7/64C02F3/32B01D53/84B01D53/62C12R1/89A23L33/00
CPCY02A50/20Y02P20/59Y02W10/37
Inventor 王媛媛李青邓平罗少敬吴洪
Owner ENN SCI & TECH DEV
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